20529

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1687426..1688042 Replicon chromosome
Accession NC_002945
Organism Mycobacterium bovis AF2122/97

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag BQ2027_RS07830 Protein ID WP_003407593.1
Coordinates 1687725..1688042 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag BQ2027_RS07825 Protein ID WP_003900349.1
Coordinates 1687426..1687728 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BQ2027_RS07815 (BQ2027_MB1529) 1683312..1685159 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
BQ2027_RS07820 (BQ2027_MB1530) 1685160..1687412 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
BQ2027_RS07825 (BQ2027_MB1531) 1687426..1687728 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
BQ2027_RS07830 (BQ2027_MB1532) 1687725..1688042 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
BQ2027_RS07835 (BQ2027_MB1533) 1688039..1689043 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
BQ2027_RS07840 (BQ2027_MB1534) 1689096..1690385 + 1290 WP_003407599.1 serine hydrolase domain-containing protein -
BQ2027_RS07845 (BQ2027_MB1535C) 1690458..1691183 - 726 WP_003898898.1 class I SAM-dependent methyltransferase -
BQ2027_RS07850 (BQ2027_MB1536C) 1691289..1691480 - 192 WP_003407609.1 dodecin family protein -
BQ2027_RS20375 (BQ2027_MB1537) 1691562..1691960 + 399 WP_003900351.1 hypothetical protein -
BQ2027_RS07860 (BQ2027_MB1538) 1692005..1693033 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T20529 WP_003407593.1 NC_002945:1687725-1688042 [Mycobacterium tuberculosis variant bovis AF2122/97]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T20529 NC_002945:1687725-1688042 [Mycobacterium tuberculosis variant bovis AF2122/97]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT20529 WP_003900349.1 NC_002945:1687426-1687728 [Mycobacterium tuberculosis variant bovis AF2122/97]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT20529 NC_002945:1687426-1687728 [Mycobacterium tuberculosis variant bovis AF2122/97]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
AlphaFold DB G0TJ73

References