Detailed information of TA system TA205288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 51996..52621
• Replicon: plasmid pSA31IncF
• Accession: NZ_CP076277
• Organism: Escherichia coli strain SA31-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A7A21_RS23965
• Protein ID: WP_000911313.1
• Coordinates: 52223..52621 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: A7A21_RS23960
• Protein ID: WP_000450520.1
• Coordinates: 51996..52223 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A21_RS23950 (47334)	47334..47759	-	426	WP_257115045.1	transposase	-
A7A21_RS23955 (47844)	47844..51914	-	4071	Protein_48	conjugative transfer relaxase/helicase TraI	-
A7A21_RS23960 (51996)	51996..52223	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
A7A21_RS23965 (52223)	52223..52621	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
A7A21_RS23970 (52630)	52630..54783	-	2154	WP_257115046.1	type IV conjugative transfer system coupling protein TraD	-
A7A21_RS23975 (54834)	54834..55574	-	741	WP_257115047.1	hypothetical protein	-
A7A21_RS23980 (55777)	55777..56487	-	711	WP_045227890.1	conjugal transfer complement resistance protein TraT	-
A7A21_RS23985 (56540)	56540..57037	-	498	WP_137546094.1	surface exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..149410	149410

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T205288 WP_000911313.1 NZ_CP076277:52223-52621 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T205288 NZ_CP101542:c4814041-4813508 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT205288 WP_000450520.1 NZ_CP076277:51996-52223 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT205288 NZ_CP101542:c4814318-4814052 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: V0VCB2