Detailed information of TA system TA205285

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 36108..36667
• Replicon: plasmid pSA31IncF
• Accession: NZ_CP076277
• Organism: Escherichia coli strain SA31-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: A7A21_RS23870
• Protein ID: WP_000421257.1
• Coordinates: 36108..36383 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: A7A21_RS23875
• Protein ID: WP_001178089.1
• Coordinates: 36383..36667 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A21_RS23835 (32505)	32505..32816	-	312	WP_001259758.1	colicin V	-
A7A21_RS23840 (32794)	32794..33030	-	237	WP_032257624.1	colicin V immunity protein	-
A7A21_RS23845 (33555)	33555..33689	-	135	WP_225381043.1	colicin release lysis protein	-
A7A21_RS23850 (33734)	33734..34948	-	1215	Protein_27	IS66-like element ISEc8 family transposase	-
A7A21_RS23855 (35105)	35105..35362	-	258	WP_000523383.1	cloacin immunity family protein	-
A7A21_RS23860 (35372)	35372..35527	-	156	Protein_29	colicin E3/pyocin S6 family cytotoxin	-
A7A21_RS23865 (35765)	35765..36013	-	249	WP_257115041.1	hypothetical protein	-
A7A21_RS23870 (36108)	36108..36383	-	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A7A21_RS23875 (36383)	36383..36667	-	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
A7A21_RS23880 (37580)	37580..38437	-	858	WP_000131011.1	incFII family plasmid replication initiator RepA	-
A7A21_RS23885 (38430)	38430..38504	-	75	WP_001365705.1	RepA leader peptide Tap	-
A7A21_RS23890 (38553)	38553..38678	+	126	WP_001436741.1	hypothetical protein	-
A7A21_RS23895 (38751)	38751..38999	-	249	WP_257115042.1	replication regulatory protein RepA	-
A7A21_RS23900 (39283)	39283..39432	-	150	WP_001300273.1	Hok/Gef family protein	-
- (39476)	39476..39537	+	62	NuclAT_1	-	-
- (39476)	39476..39537	+	62	NuclAT_1	-	-
- (39476)	39476..39537	+	62	NuclAT_1	-	-
- (39476)	39476..39537	+	62	NuclAT_1	-	-
A7A21_RS23905 (39712)	39712..39846	-	135	WP_001058542.1	hypothetical protein	-
A7A21_RS23910 (40197)	40197..40658	-	462	WP_257115043.1	thermonuclease family protein	-
A7A21_RS23915 (41401)	41401..41604	-	204	WP_001327131.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..149410	149410
	-	flank	IS/Tn	-	-	33734..34933	1199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T205285 WP_000421257.1 NZ_CP076277:c36383-36108 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T205285 NZ_CP101542:2050852-2050954 [Klebsiella pneumoniae subsp. pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT205285 WP_001178089.1 NZ_CP076277:c36667-36383 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT205285 NZ_CP101542:c2050961-2050816 [Klebsiella pneumoniae subsp. pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: A0A731IHA8