Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3848864..3849495 Replicon chromosome
Accession NZ_CP076276
Organism Escherichia coli strain SA31-1

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag A7A21_RS18975 Protein ID WP_001259388.1
Coordinates 3849220..3849495 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag A7A21_RS18970 Protein ID WP_000593555.1
Coordinates 3848864..3849223 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A21_RS18950 (3845369) 3845369..3846163 + 795 Protein_3709 PTS galactitol transporter subunit IIC -
A7A21_RS18955 (3846156) 3846156..3847664 + 1509 WP_021554579.1 FGGY-family carbohydrate kinase -
A7A21_RS18960 (3847654) 3847654..3847923 + 270 WP_021554580.1 HPr family phosphocarrier protein -
A7A21_RS18965 (3847955) 3847955..3848815 + 861 WP_021554581.1 class II aldolase -
A7A21_RS18970 (3848864) 3848864..3849223 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
A7A21_RS18975 (3849220) 3849220..3849495 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
A7A21_RS18980 (3849568) 3849568..3850692 - 1125 WP_001314210.1 ABC transporter permease -
A7A21_RS18985 (3850692) 3850692..3853427 - 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
A7A21_RS18990 (3853424) 3853424..3854491 - 1068 WP_000361485.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3841946..3858347 16401


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T205281 WP_001259388.1 NZ_CP076276:c3849495-3849220 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T205281 NZ_CP101542:329978-330346 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT205281 WP_000593555.1 NZ_CP076276:c3849223-3848864 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT205281 NZ_CP101542:329760-329981 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References