Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1574828..1575483 Replicon chromosome
Accession NC_002945
Organism Mycobacterium bovis AF2122/97

Toxin (Protein)


Gene name vapC Uniprot ID A0A7U4FAR1
Locus tag BQ2027_RS07320 Protein ID WP_003407268.1
Coordinates 1574828..1575229 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TIK2
Locus tag BQ2027_RS07325 Protein ID WP_003407272.1
Coordinates 1575226..1575483 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BQ2027_RS07305 (BQ2027_MB1429C) 1570672..1572057 - 1386 WP_003407260.1 cytochrome P450 -
BQ2027_RS07310 (BQ2027_MB1430) 1572135..1573169 + 1035 WP_003407264.1 AraC family transcriptional regulator -
BQ2027_RS07315 (BQ2027_MB1431C) 1573215..1574573 - 1359 WP_010950541.1 PE family protein -
BQ2027_RS07320 (BQ2027_MB1432C) 1574828..1575229 - 402 WP_003407268.1 type II toxin-antitoxin system VapC family toxin Toxin
BQ2027_RS07325 (BQ2027_MB1433C) 1575226..1575483 - 258 WP_003407272.1 CopG family transcriptional regulator Antitoxin
BQ2027_RS07330 (BQ2027_MB1434C) 1575566..1576525 - 960 WP_003407276.1 alpha/beta hydrolase -
BQ2027_RS07335 (BQ2027_MB1435C) 1576550..1577512 - 963 WP_003407279.1 alpha/beta hydrolase -
BQ2027_RS07340 (BQ2027_MB1436) 1577511..1578248 + 738 WP_031647000.1 lysoplasmalogenase -
BQ2027_RS07345 (BQ2027_MB1437) 1578329..1580296 + 1968 WP_003407285.1 primosomal protein N' -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-108)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-39)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14894.39 Da        Isoelectric Point: 11.6897

>T20528 WP_003407268.1 NC_002945:c1575229-1574828 [Mycobacterium tuberculosis variant bovis AF2122/97]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQGLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Download         Length: 402 bp

>T20528 NC_002945:c1575229-1574828 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGGCCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9443.58 Da        Isoelectric Point: 6.4631

>AT20528 WP_003407272.1 NC_002945:c1575483-1575226 [Mycobacterium tuberculosis variant bovis AF2122/97]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Download         Length: 258 bp

>AT20528 NC_002945:c1575483-1575226 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FAR1

Antitoxin

Source ID Structure
AlphaFold DB G0TIK2

References