Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3205372..3206026 Replicon chromosome
Accession NZ_CP076276
Organism Escherichia coli strain SA31-1

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag A7A21_RS15695 Protein ID WP_000244772.1
Coordinates 3205372..3205779 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag A7A21_RS15700 Protein ID WP_000354046.1
Coordinates 3205760..3206026 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A21_RS15675 (3201329) 3201329..3203062 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
A7A21_RS15680 (3203068) 3203068..3203778 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
A7A21_RS15685 (3203803) 3203803..3204699 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
A7A21_RS15690 (3204811) 3204811..3205332 + 522 WP_001055874.1 flavodoxin FldB -
A7A21_RS15695 (3205372) 3205372..3205779 - 408 WP_000244772.1 protein YgfX Toxin
A7A21_RS15700 (3205760) 3205760..3206026 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
A7A21_RS15705 (3206269) 3206269..3207249 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
A7A21_RS15710 (3207445) 3207445..3208104 - 660 WP_000250274.1 hemolysin III family protein -
A7A21_RS15715 (3208268) 3208268..3208579 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
A7A21_RS15720 (3208624) 3208624..3210057 + 1434 WP_157914566.1 6-phospho-beta-glucosidase BglA -
A7A21_RS15725 (3210114) 3210114..3210857 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T205278 WP_000244772.1 NZ_CP076276:c3205779-3205372 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T205278 NZ_CP101540:c4515442-4515032 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTTGACACCAACATCTGCATCTTCACTATCAAAAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGACCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATTGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGACTGGGTCGTCC
CAATCGTGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT205278 WP_000354046.1 NZ_CP076276:c3206026-3205760 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT205278 NZ_CP101540:c4515672-4515442 [Pseudomonas aeruginosa]
GTGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCCGTCGCGCTTCCTGATGACGT
TAAGCGTGTGGATGTGGTCGCTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGTTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References