Detailed information of TA system TA020526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1389957..1390516
• Replicon: chromosome
• Accession: NC_002945
• Organism: Mycobacterium bovis AF2122/97

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0THS1
• Locus tag: BQ2027_RS06520
• Protein ID: WP_003898789.1
• Coordinates: 1389957..1390250 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0THS2
• Locus tag: BQ2027_RS06525
• Protein ID: WP_003406322.1
• Coordinates: 1390247..1390516 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BQ2027_RS06495 (BQ2027_MB1273)	1385550..1385810	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	-
BQ2027_RS06500 (BQ2027_MB1274)	1385807..1386238	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	-
BQ2027_RS06505 (BQ2027_MB1275C)	1386261..1387949	-	1689	WP_003910308.1	PE family protein	-
BQ2027_RS06510 (BQ2027_MB1276)	1388129..1388989	+	861	WP_003406306.1	glycine betaine ABC transporter substrate-binding protein	-
BQ2027_RS06515 (BQ2027_MB1277C)	1389070..1389900	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
BQ2027_RS06520 (BQ2027_MB1278C)	1389957..1390250	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
BQ2027_RS06525 (BQ2027_MB1279C)	1390247..1390516	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BQ2027_RS06530 (BQ2027_MB1280C)	1390629..1394324	-	3696	WP_010950509.1	multifunctional oxoglutarate decarboxylase/oxoglutarate dehydrogenase thiamine pyrophosphate-binding subunit/dihydrolipoyllysine-residue succinyltransferase subunit	-
BQ2027_RS06535 (BQ2027_MB1281C)	1394466..1395254	-	789	WP_010950510.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11031.57 Da
• Isoelectric Point: 9.6275

>T20526 WP_003898789.1 NC_002945:c1390250-1389957 [Mycobacterium tuberculosis variant bovis AF2122/97]
VSDDHPYHVAITATAARDLQRLPEKIAAACVEFVFGPLLNNPHRLGKPLRNDLEGLHSARRGDYRVVYAIDDGHHRVEII
HIARRSASYRMNPCRPR

Nucleotide

• Length: 294 bp

>T20526 NC_002945:c1390250-1389957 [Mycobacterium tuberculosis variant bovis AF2122/97]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9766.97 Da
• Isoelectric Point: 4.8615

>AT20526 WP_003406322.1 NC_002945:c1390516-1390247 [Mycobacterium tuberculosis variant bovis AF2122/97]
MAVVPLGEVRNRLSEYVAEVELTHERITITRHGHPAAVLISADDLASIEETLEVLRTPGASEAIREGLADVAAGRFVSND
EIRNRYTAR

Nucleotide

• Length: 270 bp

>AT20526 NC_002945:c1390516-1390247 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Structures

Toxin

• AlphaFold DB: G0THS1

Antitoxin

• AlphaFold DB: G0THS2