Detailed information of TA system TA205258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 138263..138798
• Replicon: plasmid pP393F10IncF
• Accession: NZ_CP076275
• Organism: Escherichia coli strain P393-F10

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q60228
• Locus tag: A7A10_RS25940
• Protein ID: WP_001270417.1
• Coordinates: 138511..138798 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: A7A10_RS25935
• Protein ID: WP_001132895.1
• Coordinates: 138263..138514 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A10_RS25910 (135059)	135059..135316	+	258	WP_257115022.1	biofilm development regulator YmgB/AriR family protein	-
A7A10_RS25915 (135395)	135395..135661	-	267	Protein_140	IS1 family transposase	-
A7A10_RS25920 (135842)	135842..136738	-	897	Protein_141	IS3-like element IS600 family transposase	-
A7A10_RS25925 (136745)	136745..137896	-	1152	WP_257185956.1	IS30-like element IS30 family transposase	-
A7A10_RS25930 (137961)	137961..138140	-	180	Protein_143	IS3 family transposase	-
A7A10_RS25935 (138263)	138263..138514	+	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
A7A10_RS25940 (138511)	138511..138798	+	288	WP_001270417.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A7A10_RS25945 (138800)	138800..139211	+	412	Protein_146	DDE-type integrase/transposase/recombinase	-
A7A10_RS25950 (139856)	139856..140833	+	978	WP_000361612.1	RepB family plasmid replication initiator protein	-
A7A10_RS25955 (141118)	141118..141858	-	741	WP_001066942.1	tyrosine-type recombinase/integrase	-
A7A10_RS25960 (141979)	141979..142167	-	189	WP_172944323.1	serine/threonine protein kinase	-
A7A10_RS25965 (142542)	142542..143444	-	903	WP_001312822.1	antimicrobial resistance protein Mig-14	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..153041	153041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11214.24 Da
• Isoelectric Point: 10.4703

>T205258 WP_001270417.1 NZ_CP076275:138511-138798 [Escherichia coli]
MRYQVKFREDALKEWQKLDKAIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T205258 NZ_CP101533:353992-354339 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT205258 WP_001132895.1 NZ_CP076275:138263-138514 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT205258 NZ_CP101533:354339-354638 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A5T8BSB4

Antitoxin

• AlphaFold DB: L4J0V6