Detailed information of TA system TA020525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1385550..1386238
• Replicon: chromosome
• Accession: NC_002945
• Organism: Mycobacterium bovis AF2122/97

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: BQ2027_RS06500
• Protein ID: WP_003406304.1
• Coordinates: 1385807..1386238 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: BQ2027_RS06495
• Protein ID: WP_003406302.1
• Coordinates: 1385550..1385810 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BQ2027_RS06475 (BQ2027_MB1269)	1381127..1381951	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
BQ2027_RS06480 (BQ2027_MB1270)	1381956..1383137	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
BQ2027_RS06485 (BQ2027_MB1271C)	1383214..1384314	-	1101	WP_010950508.1	magnesium/cobalt transporter CorA	-
BQ2027_RS06490 (BQ2027_MB1272)	1384485..1385474	+	990	WP_003406301.1	malate dehydrogenase	-
BQ2027_RS06495 (BQ2027_MB1273)	1385550..1385810	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
BQ2027_RS06500 (BQ2027_MB1274)	1385807..1386238	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
BQ2027_RS06505 (BQ2027_MB1275C)	1386261..1387949	-	1689	WP_003910308.1	PE family protein	-
BQ2027_RS06510 (BQ2027_MB1276)	1388129..1388989	+	861	WP_003406306.1	glycine betaine ABC transporter substrate-binding protein	-
BQ2027_RS06515 (BQ2027_MB1277C)	1389070..1389900	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
BQ2027_RS06520 (BQ2027_MB1278C)	1389957..1390250	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
BQ2027_RS06525 (BQ2027_MB1279C)	1390247..1390516	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T20525 WP_003406304.1 NC_002945:1385807-1386238 [Mycobacterium tuberculosis variant bovis AF2122/97]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T20525 NC_002945:1385807-1386238 [Mycobacterium tuberculosis variant bovis AF2122/97]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT20525 WP_003406302.1 NC_002945:1385550-1385810 [Mycobacterium tuberculosis variant bovis AF2122/97]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT20525 NC_002945:1385550-1385810 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6