Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4728936..4729433 Replicon chromosome
Accession NZ_CP076274
Organism Escherichia coli strain P393-F10

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag A7A10_RS23200 Protein ID WP_001173343.1
Coordinates 4729260..4729433 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag A7A10_RS23195 Protein ID WP_000398619.1
Coordinates 4728936..4729232 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A10_RS23175 (4725667) 4725667..4726386 - 720 WP_000611288.1 two-component system response regulator DcuR -
A7A10_RS23180 (4726383) 4726383..4728014 - 1632 WP_001216461.1 sensor histidine kinase -
A7A10_RS23185 (4728195) 4728195..4728425 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
A7A10_RS23190 (4728437) 4728437..4728709 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
A7A10_RS23195 (4728936) 4728936..4729232 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
A7A10_RS23200 (4729260) 4729260..4729433 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
A7A10_RS23205 (4729552) 4729552..4731069 - 1518 WP_001295074.1 lysine--tRNA ligase -
A7A10_RS23210 (4731306) 4731306..4732763 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T205245 WP_001173343.1 NZ_CP076274:4729260-4729433 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T205245 NZ_CP101524:154693-155094 [Burkholderia arboris]
ATGCCGCGTTACATGCTCGACACCAACATGTGCATCTATCTGATCAAGAATCAGCCGGAGCAGGTCGCGAGACGGTTCGC
GCAGTGTTACAGCGGAGATGTCGTGATGTCGGCGATCACCCATGCCGAACTCGAGTACGGTGTCGCGGCGTGCGCGAATC
CTGTCCGCGAGCGTCGCAATCTTGCTGCGCTGATCGAGGACATCCCCGTTGCTCCGTTCGACACGGCGGCCGCGCAAGCA
TACGGCCCGGTTCGGGAAGCGACGCGCGAGCGGAAGAAGGATCATCTCGACAAACTGATCGCCGCGCATGCGGTGTCGCT
CGATGTGGTCCTGGTGACCAACAACGAACGGGATTTTGCGAGTTATCCGGGATTGCGGCTGGAGAACTGGTTGAACGATT
GA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT205245 WP_000398619.1 NZ_CP076274:4728936-4729232 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT205245 NZ_CP101524:154457-154693 [Burkholderia arboris]
GTGCATACCACCAGGGTATTTCGGAACGGTAACTCGCAGGCCGTGCGGATTCCTGCGGACCTTGCCTATGAGCGCAGCGA
CATGGAACTCGAAATCGAGCGGGTGGGCGACGAAATCCGTATTCGGCCCACTCGGAGACCTTTGACCCACGTACTCGAGA
AGTTCGCGAACTTCGGGCCGGATTTCATGGCCGAGGGGCGCGGCGATCAGGAGCAGGCCGAGCGCGAGGGCTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References