Detailed information of TA system TA020524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1240815..1241383
• Replicon: chromosome
• Accession: NC_002945
• Organism: Mycobacterium bovis AF2122/97

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: BQ2027_RS05855
• Protein ID: WP_003405865.1
• Coordinates: 1241009..1241383 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: BQ2027_RS05850
• Protein ID: WP_003405863.1
• Coordinates: 1240815..1241012 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BQ2027_RS05830 (BQ2027_MB1139C)	1236856..1237494	-	639	WP_003898730.1	lipid droplet-associated protein	-
BQ2027_RS05835 (BQ2027_MB1140)	1237584..1238591	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
BQ2027_RS05840 (BQ2027_MB1141C)	1238608..1239591	-	984	WP_003898731.1	hypothetical protein	-
BQ2027_RS05845 (BQ2027_MB1142)	1239654..1240727	+	1074	WP_003405860.1	redox-regulated ATPase YchF	-
BQ2027_RS05850 (BQ2027_MB1143)	1240815..1241012	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BQ2027_RS05855 (BQ2027_MB1144)	1241009..1241383	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
BQ2027_RS05860 (BQ2027_MB1145)	1241586..1242284	+	699	WP_010950494.1	hypothetical protein	-
BQ2027_RS05865 (BQ2027_MB1146)	1242402..1242587	+	186	WP_003901093.1	hypothetical protein	-
BQ2027_RS05870 (BQ2027_MB1147C)	1242514..1242789	-	276	WP_003405867.1	hypothetical protein	-
BQ2027_RS05875 (BQ2027_MB1148)	1243032..1243355	+	324	WP_003405871.1	putative quinol monooxygenase	-
BQ2027_RS05880 (BQ2027_MB1149C)	1243370..1244068	-	699	WP_031646953.1	hypothetical protein	-
BQ2027_RS05885	1244102..1244311	+	210	WP_003911400.1	hypothetical protein	-
BQ2027_RS05890 (BQ2027_MB1151C)	1244263..1245033	-	771	Protein_1181	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T20524 WP_003405865.1 NC_002945:1241009-1241383 [Mycobacterium tuberculosis variant bovis AF2122/97]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T20524 NC_002945:1241009-1241383 [Mycobacterium tuberculosis variant bovis AF2122/97]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT20524 WP_003405863.1 NC_002945:1240815-1241012 [Mycobacterium tuberculosis variant bovis AF2122/97]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT20524 NC_002945:1240815-1241012 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07