205238

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3146837..3147420 Replicon chromosome
Accession NZ_CP076274
Organism Escherichia coli strain P393-F10

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag A7A10_RS15610 Protein ID WP_257185734.1
Coordinates 3146837..3147172 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag A7A10_RS15615 Protein ID WP_000581937.1
Coordinates 3147172..3147420 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A10_RS15590 (3142306) 3142306..3142668 + 363 WP_000034929.1 hypothetical protein -
A7A10_RS15595 (3142724) 3142724..3144022 - 1299 WP_000036723.1 phosphopyruvate hydratase -
A7A10_RS15600 (3144110) 3144110..3145747 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
A7A10_RS15605 (3145975) 3145975..3146766 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
A7A10_RS15610 (3146837) 3146837..3147172 - 336 WP_257185734.1 endoribonuclease MazF Toxin
A7A10_RS15615 (3147172) 3147172..3147420 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
A7A10_RS15620 (3147498) 3147498..3149732 - 2235 WP_000226815.1 GTP pyrophosphokinase -
A7A10_RS15625 (3149780) 3149780..3151081 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12172.10 Da        Isoelectric Point: 8.4777

>T205238 WP_257185734.1 NZ_CP076274:c3147172-3146837 [Escherichia coli]
MISRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T205238 NZ_CP101518:822949-823458 [Ensifer adhaerens]
ATGGCGGTTGGTGCGCCGGTGCGCCTCTGGCGCGCCTATGTGCCGCGCTGGGCGCATGCGCCGCTCTCGGGCGAAGGGGC
AGGGCGGTACGGTGGGAGGTGGAATCCCGTGGGAACGCCGACGATCTATGCCGCCCGCGAGCTCTCGACCGCCTGGGCCG
AATACAATCAGGGTTTCGTCCAACACCCGGCGCTGATCGCCCAGCTTGAACTCCGCTTTGCCCGTCTTGCCGATCTGACG
GCGCCGGAGATCCTTTCCGACCTTGGTCTCACCGAAGAAATCCACCGATGCGAATGGCGCGATGCGCTCGATCGTGGTCT
GGTGCCGGAGACGCACCGCCTGCAACTGGCGTTGAGCGACCGAGGCTTCGATGGCCTGATCTATCCGTCCTTCATGTCGC
CCGGCGGCACCTGCGTCGCGCTGTGGCACTGGAACGCGGGCGAGGGTTCCGAACTGAAGATTATCGATCCCGAAGGGCGC
CTGCCGAAAACGCCGGCGTCCTGGCTCTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT205238 WP_000581937.1 NZ_CP076274:c3147420-3147172 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT205238 NZ_CP101518:822578-822949 [Ensifer adhaerens]
ATGGCTGCTGTCGGTCTCAACATCTCCACCGCCCACTACGGCGAGAGCCATTCGTCTTTCCTGTCCGCTCGGCTGGTTGC
CGATCGCCTTGGCGTGACGCTTGCCGAACTCGCGAAGCTGATCGGTGTCGCGCGCAACACGCTGACGGCGAAGTCCAGTG
CCCGAAAGGTCGATGCGGCGTTGAGCAGCGTCGTCCGTATCCTTGCCATGGCCGGCGAAATGGCGGGCGACGACAGCCGC
GCCGTCATCTGGTTCAAGCACCAGCCGATCCCCGGCTGGGCCGGCAAGACCGCCTATGATCTCGTCGGCGAGGGCAAGGC
CGACAAGGTGCTCGCCTATCTCGAAGCCGTGCGTTCCGGTGTCTACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References