Detailed information of TA system TA205238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3146837..3147420
• Replicon: chromosome
• Accession: NZ_CP076274
• Organism: Escherichia coli strain P393-F10

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: A7A10_RS15610
• Protein ID: WP_257185734.1
• Coordinates: 3146837..3147172 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S1P3W5
• Locus tag: A7A10_RS15615
• Protein ID: WP_000581937.1
• Coordinates: 3147172..3147420 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A10_RS15590 (3142306)	3142306..3142668	+	363	WP_000034929.1	hypothetical protein	-
A7A10_RS15595 (3142724)	3142724..3144022	-	1299	WP_000036723.1	phosphopyruvate hydratase	-
A7A10_RS15600 (3144110)	3144110..3145747	-	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
A7A10_RS15605 (3145975)	3145975..3146766	-	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
A7A10_RS15610 (3146837)	3146837..3147172	-	336	WP_257185734.1	endoribonuclease MazF	Toxin
A7A10_RS15615 (3147172)	3147172..3147420	-	249	WP_000581937.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
A7A10_RS15620 (3147498)	3147498..3149732	-	2235	WP_000226815.1	GTP pyrophosphokinase	-
A7A10_RS15625 (3149780)	3149780..3151081	-	1302	WP_000046790.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12172.10 Da
• Isoelectric Point: 8.4777

>T205238 WP_257185734.1 NZ_CP076274:c3147172-3146837 [Escherichia coli]
MISRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T205238 NZ_CP101518:822949-823458 [Ensifer adhaerens]
ATGGCGGTTGGTGCGCCGGTGCGCCTCTGGCGCGCCTATGTGCCGCGCTGGGCGCATGCGCCGCTCTCGGGCGAAGGGGC
AGGGCGGTACGGTGGGAGGTGGAATCCCGTGGGAACGCCGACGATCTATGCCGCCCGCGAGCTCTCGACCGCCTGGGCCG
AATACAATCAGGGTTTCGTCCAACACCCGGCGCTGATCGCCCAGCTTGAACTCCGCTTTGCCCGTCTTGCCGATCTGACG
GCGCCGGAGATCCTTTCCGACCTTGGTCTCACCGAAGAAATCCACCGATGCGAATGGCGCGATGCGCTCGATCGTGGTCT
GGTGCCGGAGACGCACCGCCTGCAACTGGCGTTGAGCGACCGAGGCTTCGATGGCCTGATCTATCCGTCCTTCATGTCGC
CCGGCGGCACCTGCGTCGCGCTGTGGCACTGGAACGCGGGCGAGGGTTCCGAACTGAAGATTATCGATCCCGAAGGGCGC
CTGCCGAAAACGCCGGCGTCCTGGCTCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9355.74 Da
• Isoelectric Point: 4.4665

>AT205238 WP_000581937.1 NZ_CP076274:c3147420-3147172 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT205238 NZ_CP101518:822578-822949 [Ensifer adhaerens]
ATGGCTGCTGTCGGTCTCAACATCTCCACCGCCCACTACGGCGAGAGCCATTCGTCTTTCCTGTCCGCTCGGCTGGTTGC
CGATCGCCTTGGCGTGACGCTTGCCGAACTCGCGAAGCTGATCGGTGTCGCGCGCAACACGCTGACGGCGAAGTCCAGTG
CCCGAAAGGTCGATGCGGCGTTGAGCAGCGTCGTCCGTATCCTTGCCATGGCCGGCGAAATGGCGGGCGACGACAGCCGC
GCCGTCATCTGGTTCAAGCACCAGCCGATCCCCGGCTGGGCCGGCAAGACCGCCTATGATCTCGTCGGCGAGGGCAAGGC
CGACAAGGTGCTCGCCTATCTCGAAGCCGTGCGTTCCGGTGTCTACGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LMB4