Detailed information of TA system TA205237

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2851428..2852053
• Replicon: chromosome
• Accession: NZ_CP076274
• Organism: Escherichia coli strain P393-F10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A7A10_RS14170
• Protein ID: WP_000911330.1
• Coordinates: 2851428..2851826 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: A7A10_RS14175
• Protein ID: WP_000450524.1
• Coordinates: 2851826..2852053 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A10_RS14150 (2847306)	2847306..2847506	+	201	WP_000383836.1	YpfN family protein	-
A7A10_RS14155 (2847616)	2847616..2848314	-	699	WP_000679823.1	esterase	-
A7A10_RS14160 (2848388)	2848388..2850403	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
A7A10_RS14165 (2850418)	2850418..2851281	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
A7A10_RS14170 (2851428)	2851428..2851826	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
A7A10_RS14175 (2851826)	2851826..2852053	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
A7A10_RS14180 (2852207)	2852207..2852920	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
A7A10_RS14185 (2853133)	2853133..2854167	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
A7A10_RS14190 (2854184)	2854184..2855062	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
A7A10_RS14195 (2855208)	2855208..2855780	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
A7A10_RS14200 (2855780)	2855780..2856250	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T205237 WP_000911330.1 NZ_CP076274:c2851826-2851428 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T205237 NZ_CP101518:c17815-17408 [Ensifer adhaerens]
ATGTTGAAATACATGCTCGACACCAATATCTGCATCTACACGGTCAAGAACCGGCCGCAGCAGGTTCGTGAGGCGTTCAA
CAGGCATCACGGCCAACTGTGCATCAGCTCGGTCAGCCTGATGGAGTTGATCTACGGCGCGGAAAGATCCGCAATGCCCG
AGCGTAACCTATCGGTCATAGAGGGGTTTGCCGCGCGTCTTGAGGTTCTCAACTACGATCAGGCGGCAGCCAGCCATACC
GGACAGTTGCGCGCCGAACTCGCCCGCAGTGGCACGCCGATAGGGCCTTACGATCAGTTGATCGCGGGTCATGCACGGTC
TCAAGGCTTGATCCTCGTGACCAACAACCGGCGGGAGTTCGATCGTGTGCCCGGCTTGCGAGTCGAGGACTGGACCGCGG
CGCCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT205237 WP_000450524.1 NZ_CP076274:c2852053-2851826 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT205237 NZ_CP101518:c18048-17815 [Ensifer adhaerens]
ATGTCGGAACAGGGCGCAGTTTTTCTCACCAATCGTAGTCAGGCGATCCGGCTGCCGAGGGCCGTAGCGCTTCCGGAGGA
CGTCAAACGCGTCGATATCGTCGCGGTGGGGCGAACGCGCATCATTACACCCGCAGGCGAGGCCTGGGACAGTTGGTTCG
ACGGCGAGCTCGTCACAGCCGATTTCATGGTGCAGAGAGAGCAGCCGGAGGCCCAGGAACGCGAGCCACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33