20523

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1232059..1232690 Replicon chromosome
Accession NC_002945
Organism Mycobacterium bovis AF2122/97

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag BQ2027_RS05800 Protein ID WP_003405820.1
Coordinates 1232059..1232370 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag BQ2027_RS05805 Protein ID WP_003405836.1
Coordinates 1232370..1232690 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BQ2027_RS05780 (BQ2027_MB1128C) 1227541..1228965 - 1425 WP_003405805.1 class II fumarate hydratase -
BQ2027_RS05785 (BQ2027_MB1129C) 1228996..1230084 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
BQ2027_RS05790 (BQ2027_MB1130) 1230140..1230784 + 645 WP_003915494.1 DUF4245 domain-containing protein -
BQ2027_RS05795 (BQ2027_MB1131C) 1230791..1231947 - 1157 Protein_1162 AI-2E family transporter -
BQ2027_RS05800 (BQ2027_MB1132C) 1232059..1232370 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
BQ2027_RS05805 (BQ2027_MB1133C) 1232370..1232690 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
BQ2027_RS05810 1232700..1234236 + 1537 Protein_1165 carboxylesterase/lipase family protein -
BQ2027_RS05815 (BQ2027_MB1136C) 1234243..1235355 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
BQ2027_RS05820 (BQ2027_MB1137C) 1235365..1235622 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
BQ2027_RS05825 (BQ2027_MB1138C) 1235612..1236859 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
BQ2027_RS05830 (BQ2027_MB1139C) 1236856..1237494 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T20523 WP_003405820.1 NC_002945:c1232370-1232059 [Mycobacterium tuberculosis variant bovis AF2122/97]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T20523 NC_002945:c1232370-1232059 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT20523 WP_003405836.1 NC_002945:c1232690-1232370 [Mycobacterium tuberculosis variant bovis AF2122/97]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT20523 NC_002945:c1232690-1232370 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References