Detailed information of TA system TA205227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1771493..1773081
• Replicon: chromosome
• Accession: NZ_CP076274
• Organism: Escherichia coli strain P393-F10

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U9XU38
• Locus tag: A7A10_RS08795
• Protein ID: WP_001125455.1
• Coordinates: 1771493..1772815 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: A7A10_RS08800
• Protein ID: WP_001296726.1
• Coordinates: 1772815..1773081 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A10_RS08785 (1768309)	1768309..1769859	-	1551	WP_097290623.1	site-specific integrase	-
A7A10_RS08790 (1769843)	1769843..1771243	-	1401	WP_001388361.1	site-specific integrase	-
A7A10_RS08795 (1771493)	1771493..1772815	-	1323	WP_001125455.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
A7A10_RS08800 (1772815)	1772815..1773081	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
A7A10_RS08805 (1773290)	1773290..1777332	-	4043	Protein_1724	ECSE_1600 family autotransporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49321.93 Da
• Isoelectric Point: 8.8753

>T205227 WP_001125455.1 NZ_CP076274:c1772815-1771493 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T205227 NZ_CP101513:2642045-2642152 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT205227 WP_001296726.1 NZ_CP076274:c1773081-1772815 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT205227 NZ_CP101513:c2641998-2641932 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: U9XU38