205226

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1648311..1648949 Replicon chromosome
Accession NZ_CP076274
Organism Escherichia coli strain P393-F10

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag A7A10_RS08265 Protein ID WP_000813794.1
Coordinates 1648311..1648487 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag A7A10_RS08270 Protein ID WP_001270286.1
Coordinates 1648533..1648949 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A10_RS08245 (1643930) 1643930..1645144 - 1215 WP_001349372.1 BenE family transporter YdcO -
A7A10_RS08250 (1645197) 1645197..1645733 + 537 WP_000429141.1 DNA-binding transcriptional regulator SutR -
A7A10_RS08255 (1645806) 1645806..1647767 + 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
A7A10_RS08260 (1647859) 1647859..1648089 - 231 WP_000494244.1 YncJ family protein -
A7A10_RS08265 (1648311) 1648311..1648487 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
A7A10_RS08270 (1648533) 1648533..1648949 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
A7A10_RS08275 (1649028) 1649028..1650434 + 1407 WP_000760629.1 PLP-dependent aminotransferase family protein -
A7A10_RS08280 (1650679) 1650679..1651824 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
A7A10_RS08285 (1651842) 1651842..1652855 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
A7A10_RS08290 (1652856) 1652856..1653797 + 942 WP_257185878.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1642625..1643890 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T205226 WP_000813794.1 NZ_CP076274:1648311-1648487 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T205226 NZ_CP101513:2641510-2641617 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT205226 WP_001270286.1 NZ_CP076274:1648533-1648949 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT205226 NZ_CP101513:c2641463-2641397 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References