Detailed information of TA system TA205216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 468479..469316
• Replicon: chromosome
• Accession: NZ_CP076274
• Organism: Escherichia coli strain P393-F10

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: A7A10_RS02135
• Protein ID: WP_000227784.1
• Coordinates: 468479..469021 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: A7A10_RS02140
• Protein ID: WP_001297137.1
• Coordinates: 469005..469316 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A10_RS02115 (464018)	464018..464929	-	912	WP_000705847.1	2-dehydropantoate 2-reductase	-
A7A10_RS02120 (465097)	465097..465588	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
A7A10_RS02125 (465716)	465716..467080	-	1365	WP_001000978.1	MFS transporter	-
A7A10_RS02130 (467488)	467488..468423	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
A7A10_RS02135 (468479)	468479..469021	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
A7A10_RS02140 (469005)	469005..469316	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
A7A10_RS02145 (469501)	469501..470391	-	891	WP_000971336.1	heme o synthase	-
A7A10_RS02150 (470403)	470403..470732	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
A7A10_RS02155 (470732)	470732..471346	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
A7A10_RS02160 (471336)	471336..473327	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
A7A10_RS02165 (473349)	473349..474296	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T205216 WP_000227784.1 NZ_CP076274:c469021-468479 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T205216 NZ_CP101513:667646-667960 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT205216 WP_001297137.1 NZ_CP076274:c469316-469005 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT205216 NZ_CP101513:667957-668373 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3