Detailed information of TA system TA205213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 4843332..4844667
• Replicon: chromosome
• Accession: NZ_CP076272
• Organism: Escherichia coli strain E21845/O/A

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: A7A11_RS23680
• Protein ID: WP_115427420.1
• Coordinates: 4843720..4844667 (+)
• Length: 316 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: E0J2C6
• Locus tag: A7A11_RS23675
• Protein ID: WP_000152741.1
• Coordinates: 4843332..4843727 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A11_RS23660 (4839367)	4839367..4840572	-	1206	WP_074598901.1	restriction endonuclease subunit S	-
A7A11_RS23665 (4840569)	4840569..4842123	-	1555	Protein_4617	type I restriction-modification system subunit M	-
A7A11_RS23670 (4842429)	4842429..4843262	+	834	WP_001192742.1	DUF4942 domain-containing protein	-
A7A11_RS23675 (4843332)	4843332..4843727	+	396	WP_000152741.1	DUF6088 family protein	Antitoxin
A7A11_RS23680 (4843720)	4843720..4844667	+	948	WP_115427420.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
A7A11_RS23685 (4844705)	4844705..4844890	-	186	WP_000066585.1	hypothetical protein	-
A7A11_RS23690 (4844989)	4844989..4845132	-	144	Protein_4622	HNH endonuclease	-
A7A11_RS23695 (4845269)	4845269..4845919	+	651	WP_001037963.1	HNH endonuclease	-
A7A11_RS23700 (4845927)	4845927..4846907	-	981	WP_000991446.1	sialate O-acetylesterase	-
A7A11_RS23705 (4846972)	4846972..4848078	-	1107	WP_001298927.1	N-acetylneuraminate epimerase	-
A7A11_RS23710 (4848098)	4848098..4848814	-	717	WP_001295734.1	N-acetylneuraminic acid outer membrane channel NanC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimB / fimE	4821240..4851945	30705

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-303)

Antitoxin

• Protein of unknown function DUF6088 (7-108)

Sequences

Toxin

Protein

• Length: 316 a.a.
• Molecular weight: 35592.78 Da
• Isoelectric Point: 4.9737

>T205213 WP_115427420.1 NZ_CP076272:4843720-4844667 [Escherichia coli]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYTQFCASPVDELKMGLDELANNPLYQMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA

Nucleotide

• Length: 948 bp

>T205213 NZ_CP101506:544005-544355 [Bacillus altitudinis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACAATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14619.93 Da
• Isoelectric Point: 11.6915

>AT205213 WP_000152741.1 NZ_CP076272:4843332-4843727 [Escherichia coli]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRELVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG

Nucleotide

• Length: 396 bp

>AT205213 NZ_CP101506:543719-544000 [Bacillus altitudinis]
TTGTCTGAATCCAGCGCAACAAATGAAATGAAGATTCGACTGCCGGAGGCTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1L4ZXD4