205208

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3912223..3913023 Replicon chromosome
Accession NZ_CP076272
Organism Escherichia coli strain E21845/O/A

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag A7A11_RS19360 Protein ID WP_000342449.1
Coordinates 3912223..3912750 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag A7A11_RS19365 Protein ID WP_001277108.1
Coordinates 3912757..3913023 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A11_RS19335 (3907298) 3907298..3908065 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
A7A11_RS19340 (3908062) 3908062..3909339 - 1278 WP_047659341.1 branched chain amino acid ABC transporter permease LivM -
A7A11_RS19345 (3909336) 3909336..3910262 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
A7A11_RS19350 (3910310) 3910310..3911419 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
A7A11_RS19355 (3911843) 3911843..3912226 + 384 WP_000778785.1 aspartate 1-decarboxylase autocleavage activator PanM -
A7A11_RS19360 (3912223) 3912223..3912750 - 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
A7A11_RS19365 (3912757) 3912757..3913023 - 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
A7A11_RS19370 (3913173) 3913173..3914276 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
A7A11_RS19375 (3914547) 3914547..3915401 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
A7A11_RS19380 (3915646) 3915646..3916704 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
A7A11_RS19385 (3916697) 3916697..3917365 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T205208 WP_000342449.1 NZ_CP076272:c3912750-3912223 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T205208 NZ_CP101497:205794-206045 [Microcella sp. MMS21-STM10]
ATGACTGCCATTAGCGCCACCGAAGCGCGTAAGACGCTCTTCGGGCTCATCCAGCAGGTCAACGACGACCACACCGCGGT
GGAGGTCGTCTCCAAGCACGGCAACGCGGTCATTCTCTCAAAGGCCGACTACGACGCCATGACCGAGACGGCGTACCTGC
TGCGCAACCCGGCGAGCGCCGAGCGACTGCTCGCCTCGATCGAGCGCGCGCGCCGTGGAGAGTTCGAGCAGCACACCCTC
ACCGACGACTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT205208 WP_001277108.1 NZ_CP076272:c3913023-3912757 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT205208 NZ_CP101497:206059-206319 [Microcella sp. MMS21-STM10]
ATGAGTCGCACCGTCGCCTTCGATCCGCACGGGTGGGAGGACTACGTCTACTGGCAGTCCCAAGATCGCAAGACGCTCAA
GCGCATCAATCTGCTCATCACCGACGCTCTGCGTGACCCGTTCGCGGGCATCGGCAAGCCAGAGCCGCTCAAGCACATCC
TGTCGGGCGCGTGGTCACGGCGCGTCGACGACGTGAACCGCCTCGTCTACTTCGTGACCGACGACCACATCGTGATCCTG
CAGGCCCGCCAGCACTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References