205205

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3199625..3200208 Replicon chromosome
Accession NZ_CP076272
Organism Escherichia coli strain E21845/O/A

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag A7A11_RS15860 Protein ID WP_000254745.1
Coordinates 3199625..3199960 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag A7A11_RS15865 Protein ID WP_000581937.1
Coordinates 3199960..3200208 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A11_RS15845 (3195512) 3195512..3196810 - 1299 WP_000036723.1 phosphopyruvate hydratase -
A7A11_RS15850 (3196898) 3196898..3198535 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
A7A11_RS15855 (3198763) 3198763..3199554 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
A7A11_RS15860 (3199625) 3199625..3199960 - 336 WP_000254745.1 endoribonuclease MazF Toxin
A7A11_RS15865 (3199960) 3199960..3200208 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
A7A11_RS15870 (3200286) 3200286..3202520 - 2235 WP_000226815.1 GTP pyrophosphokinase -
A7A11_RS15875 (3202568) 3202568..3203869 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T205205 WP_000254745.1 NZ_CP076272:c3199960-3199625 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T205205 NZ_CP101472:933001-933363 [Staphylococcus equorum]
ATGATGAGAAGAGGAGACGTTTATTTAGCTGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAATAAGACCTGTCGT
TATCATACAGAATGATACTGGAAATAAATATAGTCCTACCGTAATTGTAGCTGCAATTACAGGTAGGATAAATAAAGCTA
AAATACCTACACATGTAGAAATAGAAAAAAGTAAATATAAGCTTGATAAAGATTCTGTAATACTGCTTGAGCAGATTAGA
ACTTTAGATAAAAATAGGCTGAAAGAAAAATTAACATACCTTTCTGAAAGTAAAATGAAAGAAGTTAATGTTGCCATAGA
TATAAGCTTAGGATTACATAATGTTAGAAATAGTAAATCATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT205205 WP_000581937.1 NZ_CP076272:c3200208-3199960 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT205205 NZ_CP101472:932834-933004 [Staphylococcus equorum]
ATGGCAAGTTTTAATCAAAGCAGAAACCATAGTATTGAACAACTATTAAAAGAAGGCTATTCACAAATGGCCGATTTAAA
TCTCTCCCTTGCAGCAGAAGCATTTCCATTTGAATGTGAAGCTTGTGATTGCAATGAAGTTTACCTCAGCTCTAAGAATA
ACAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References