Detailed information of TA system TA205204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2862469..2863094
• Replicon: chromosome
• Accession: NZ_CP076272
• Organism: Escherichia coli strain E21845/O/A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A7A11_RS14240
• Protein ID: WP_257191614.1
• Coordinates: 2862469..2862867 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: A7A11_RS14245
• Protein ID: WP_000450524.1
• Coordinates: 2862867..2863094 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A11_RS14220 (2858376)	2858376..2858576	+	201	WP_000383836.1	YpfN family protein	-
A7A11_RS14225 (2858657)	2858657..2859355	-	699	WP_000679812.1	esterase	-
A7A11_RS14230 (2859429)	2859429..2861444	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
A7A11_RS14235 (2861459)	2861459..2862322	-	864	WP_001267507.1	neutral zinc metallopeptidase	-
A7A11_RS14240 (2862469)	2862469..2862867	-	399	WP_257191614.1	type II toxin-antitoxin system VapC family toxin	Toxin
A7A11_RS14245 (2862867)	2862867..2863094	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
A7A11_RS14250 (2863248)	2863248..2863961	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
A7A11_RS14255 (2864174)	2864174..2865208	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
A7A11_RS14260 (2865225)	2865225..2866103	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
A7A11_RS14265 (2866249)	2866249..2866821	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
A7A11_RS14270 (2866821)	2866821..2867291	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14952.29 Da
• Isoelectric Point: 8.5325

>T205204 WP_257191614.1 NZ_CP076272:c2862867-2862469 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRVELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T205204 NZ_CP101472:921673-921885 [Staphylococcus equorum]
GTGGTAACAAAAGTTATTTATATTTCCGGTATAGAAAATGAAACACAACAAAAACATATTGAACAGCAGCTTAAGCAATT
ACACGGTGTACACCAAGTCAACATAGAAATAGAAAGCAATAATGGCAAAATTTTCATTGATTTTGAAACCCCTGCAAGTT
TAAATAATTTAGAAAAAGAAGTTTATGATTTAGGATATGAAATTTTATATTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT205204 WP_000450524.1 NZ_CP076272:c2863094-2862867 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT205204 NZ_CP101472:921365-921658 [Staphylococcus equorum]
ATGGCGGAAATACAAAAAGCGCATCACTCAGAACAAATTAAATCTAATTTAAAATCAAGATTAAATCGTATAGAAGGCCA
AGTGAGAGCAATTAATCGCATGGTAGATGAAGATGTTTATTGTGATGACGTGCTCACACAAATTAGAGCCACTCGATCGG
CTTTAAATGGTGTTGCTACTAAATTATTAGACCATCATATGAAAAGTTGTATTATGGAAAAAGTAGAGCAGGGTCAACAA
GAAGAAGCTATGGAAGAACTATTGGTTACATTTCAAAAATTAATGAAGGATTAG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33