Detailed information of TA system TA020520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1015147..1015766
• Replicon: chromosome
• Accession: NC_002945
• Organism: Mycobacterium bovis AF2122/97

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: BQ2027_RS04775
• Protein ID: WP_003404726.1
• Coordinates: 1015332..1015766 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: BQ2027_RS04770
• Protein ID: WP_003404724.1
• Coordinates: 1015147..1015326 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BQ2027_RS04755 (BQ2027_MB0931)	1010620..1012200	+	1581	WP_011799139.1	serine hydrolase	-
BQ2027_RS04760 (BQ2027_MB0932)	1012197..1014590	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
BQ2027_RS04765	1014863..1015021	+	159	WP_003404720.1	hypothetical protein	-
BQ2027_RS04770 (BQ2027_MB0933)	1015147..1015326	+	180	WP_003404724.1	antitoxin	Antitoxin
BQ2027_RS04775 (BQ2027_MB0934)	1015332..1015766	+	435	WP_003404726.1	SRPBCC family protein	Toxin
BQ2027_RS04780 (BQ2027_MB0935)	1015864..1016637	+	774	WP_003404735.1	VOC family protein	-
BQ2027_RS04785 (BQ2027_MB0936)	1016702..1017151	+	450	WP_003404738.1	hypothetical protein	-
BQ2027_RS04790	1017286..1017516	-	231	WP_003898642.1	hypothetical protein	-
BQ2027_RS04795 (BQ2027_MB0937C)	1017683..1019191	-	1509	WP_010950467.1	carotenoid oxygenase family protein	-
BQ2027_RS04800 (BQ2027_MB0938C)	1019193..1020431	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T20520 WP_003404726.1 NC_002945:1015332-1015766 [Mycobacterium tuberculosis variant bovis AF2122/97]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T20520 NC_002945:1015332-1015766 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT20520 WP_003404724.1 NC_002945:1015147-1015326 [Mycobacterium tuberculosis variant bovis AF2122/97]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT20520 NC_002945:1015147-1015326 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCAGGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: G0TFQ5