Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 513106..513943 Replicon chromosome
Accession NZ_CP076272
Organism Escherichia coli strain E21845/O/A

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag A7A11_RS02400 Protein ID WP_000227784.1
Coordinates 513106..513648 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag A7A11_RS02405 Protein ID WP_001297137.1
Coordinates 513632..513943 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A11_RS02375 (508463) 508463..508954 + 492 WP_001138904.1 nucleotide binding protein YajQ -
A7A11_RS02380 (509082) 509082..510446 - 1365 WP_001000978.1 MFS transporter -
A7A11_RS02385 (510794) 510794..510955 + 162 Protein_468 sel1 repeat family protein -
A7A11_RS02395 (512253) 512253..513050 + 798 WP_226397351.1 tetratricopeptide repeat protein -
A7A11_RS02400 (513106) 513106..513648 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
A7A11_RS02405 (513632) 513632..513943 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
A7A11_RS02410 (514128) 514128..515018 - 891 WP_000971336.1 heme o synthase -
A7A11_RS02415 (515030) 515030..515359 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
A7A11_RS02420 (515359) 515359..515973 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
A7A11_RS02425 (515963) 515963..517954 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
A7A11_RS02430 (517976) 517976..518923 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T205187 WP_000227784.1 NZ_CP076272:c513648-513106 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T205187 NZ_CP101416:c3482915-3482547 [Pseudanabaena sp. Chao 1811]
ATGCCTAATGGAACCTTAACCTATAAGCGAGGAGAAATATGGTGGGTTGATCTGAACCCAACTATCGGCAGTGAGACTGA
CAAAGAACGTCCTTGCTTAATTTTGCAAAATGACATTGGCAATCAAAATGGATTAACCATCATAGTCGCGCCATTACTGC
CTAACAAAAAAACTTATCCATTTGTTGTAAATGTTGTACCGACTCCGAAGAATGGACTAAGTGGCGATCGCCATATTAAT
TTAAGCCAAATGAGGTCTGTAGATGCTCGAAGAATCAAGAATAAACAGGGCGTGTTAGAAGACATTTATTGGAAGGAAAT
TGAGAAAGCTGTATCTATTGAGCTTGGTTTCAATAACTTCTCATCTTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT205187 WP_001297137.1 NZ_CP076272:c513943-513632 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT205187 NZ_CP101416:c3483132-3482908 [Pseudanabaena sp. Chao 1811]
ATGAGTAAAAAAGTCAGCATCACCTTGGATGACGAAGTTCTCAGCTTTGTTGATCGGTTAGCAAGTAATCGTAGTAGTTT
TATCAACGATATCCTCTTAAAAGAGAAGGAGAGAATTTTCATAAAAGAACTGGAAGATGCTTATAAAGAACAAGCAAATG
ATCCAATATTTCAAGAAGAGATTTCTGTTTGGGACATTACAGTGGAAGATGGTTTAAATGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References