Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 100244..100887 Replicon plasmid pCeylon31IncF
Accession NZ_CP076271
Organism Escherichia coli strain Ceylon31

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag A7A14_RS24280 Protein ID WP_001044768.1
Coordinates 100471..100887 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag A7A14_RS24275 Protein ID WP_001261287.1
Coordinates 100244..100474 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A14_RS24265 (95423) 95423..96556 - 1134 WP_000545983.1 DUF3800 domain-containing protein -
A7A14_RS24270 (96819) 96819..99938 - 3120 WP_257122487.1 hypothetical protein -
A7A14_RS24275 (100244) 100244..100474 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
A7A14_RS24280 (100471) 100471..100887 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
A7A14_RS24285 (101049) 101049..103187 - 2139 WP_000350635.1 AAA family ATPase -
A7A14_RS24290 (103541) 103541..103798 + 258 WP_000371887.1 hypothetical protein -
A7A14_RS24295 (103798) 103798..104388 + 591 WP_000194548.1 hypothetical protein -
A7A14_RS24300 (104406) 104406..104753 - 348 WP_000142443.1 DUF6404 family protein -
A7A14_RS24305 (104882) 104882..105217 + 336 WP_057698418.1 colicin E1 family microcin immunity protein -
A7A14_RS24310 (105239) 105239..105547 - 309 WP_057698417.1 colicin-like pore-forming protein -
A7A14_RS24315 (105593) 105593..105745 - 153 WP_244566948.1 colicin Ia central receptor-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - pefD / pefC / pefA 1..127140 127140


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T205185 WP_001044768.1 NZ_CP076271:100471-100887 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T205185 NZ_CP101416:c1339164-1338901 [Pseudanabaena sp. Chao 1811]
ATGAGGAACTTTGAATATGATGAAAATAAGAGTCAATCTAATTTTGCTAAACATGGAATTGACTTTGTTGAAGCTCAAAA
ACTATGGAACGATCCAAATTTGCTAGAAATCCCTAGTAGAATTCAAGATGAACCGCGATTTGTAGTTATTGGTAAAATAA
ATAATAAACATTGGTCTGGAGTTATCACCTACCGCGATCAAAATATTCGGATCATATCTGTCCGCCGATCACGCACACAA
GAGGTTGCCCTTTATGAACGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT205185 WP_001261287.1 NZ_CP076271:100244-100474 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT205185 NZ_CP101416:c1338911-1338678 [Pseudanabaena sp. Chao 1811]
ATGAACGCTAAAGACTTTGATCAAAAATTCGAGGATGGGGAAGAAGATATTATCGAAATGCTCGATCTATCTCAAGCTAA
ACGTCCCTTAGCTGCTCAAAAAAGGCTTAGCATAGAGTTACCTATTTGGATGATTGAGCTAATTAATCAAGAAGCCAAAC
GTTTAGGAGTAACACCACAAACAATTGTCCAAACCTCTCTTGCTGAACATTTAGCAGTTAGTAACTTCAATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References