Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3848213..3848844 Replicon chromosome
Accession NZ_CP076270
Organism Escherichia coli strain Ceylon31

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag A7A14_RS18805 Protein ID WP_001259388.1
Coordinates 3848569..3848844 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag A7A14_RS18800 Protein ID WP_000593555.1
Coordinates 3848213..3848572 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A14_RS18775 (3844345) 3844345..3845289 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
A7A14_RS18780 (3845286) 3845286..3846119 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
A7A14_RS18785 (3846119) 3846119..3846883 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
A7A14_RS18790 (3846880) 3846880..3847686 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
A7A14_RS18795 (3847692) 3847692..3848093 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
A7A14_RS18800 (3848213) 3848213..3848572 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
A7A14_RS18805 (3848569) 3848569..3848844 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
A7A14_RS18810 (3848917) 3848917..3850041 - 1125 WP_257122484.1 ABC transporter permease -
A7A14_RS18815 (3850041) 3850041..3852776 - 2736 WP_000149124.1 ribosome-associated ATPase/putative transporter RbbA -
A7A14_RS18820 (3852773) 3852773..3853840 - 1068 WP_000361467.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T205175 WP_001259388.1 NZ_CP076270:c3848844-3848569 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T205175 NZ_CP101390:c4781866-4781555 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT205175 WP_000593555.1 NZ_CP076270:c3848572-3848213 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT205175 NZ_CP101390:c4781554-4781264 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References