Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3177902..3178556 Replicon chromosome
Accession NZ_CP076270
Organism Escherichia coli strain Ceylon31

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag A7A14_RS15465 Protein ID WP_000244772.1
Coordinates 3177902..3178309 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag A7A14_RS15470 Protein ID WP_000354046.1
Coordinates 3178290..3178556 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A14_RS15445 (3173859) 3173859..3175592 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
A7A14_RS15450 (3175598) 3175598..3176308 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
A7A14_RS15455 (3176333) 3176333..3177229 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
A7A14_RS15460 (3177341) 3177341..3177862 + 522 WP_001055874.1 flavodoxin FldB -
A7A14_RS15465 (3177902) 3177902..3178309 - 408 WP_000244772.1 protein YgfX Toxin
A7A14_RS15470 (3178290) 3178290..3178556 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
A7A14_RS15475 (3178799) 3178799..3179779 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
A7A14_RS15480 (3179856) 3179856..3180515 - 660 WP_000250274.1 hemolysin III family protein -
A7A14_RS15485 (3180679) 3180679..3180990 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
A7A14_RS15490 (3181035) 3181035..3182468 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
A7A14_RS15495 (3182525) 3182525..3183268 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T205172 WP_000244772.1 NZ_CP076270:c3178309-3177902 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T205172 NZ_CP101390:c4196136-4195849 [Salmonella enterica]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT205172 WP_000354046.1 NZ_CP076270:c3178556-3178290 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT205172 NZ_CP101390:c4195862-4195560 [Salmonella enterica]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References