Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3035988..3036571 Replicon chromosome
Accession NZ_CP076270
Organism Escherichia coli strain Ceylon31

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag A7A14_RS14825 Protein ID WP_257122409.1
Coordinates 3035988..3036323 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag A7A14_RS14830 Protein ID WP_000581937.1
Coordinates 3036323..3036571 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A14_RS14805 (3031457) 3031457..3031819 + 363 WP_000034929.1 hypothetical protein -
A7A14_RS14810 (3031875) 3031875..3033173 - 1299 WP_000036723.1 phosphopyruvate hydratase -
A7A14_RS14815 (3033261) 3033261..3034898 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
A7A14_RS14820 (3035126) 3035126..3035917 - 792 WP_001071643.1 nucleoside triphosphate pyrophosphohydrolase -
A7A14_RS14825 (3035988) 3035988..3036323 - 336 WP_257122409.1 endoribonuclease MazF Toxin
A7A14_RS14830 (3036323) 3036323..3036571 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
A7A14_RS14835 (3036649) 3036649..3038883 - 2235 WP_000226815.1 GTP pyrophosphokinase -
A7A14_RS14840 (3038931) 3038931..3040232 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12128.07 Da        Isoelectric Point: 8.2618

>T205171 WP_257122409.1 NZ_CP076270:c3036323-3035988 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPTVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T205171 NZ_CP101390:3784901-3785176 [Salmonella enterica]
ATGAATGCCTCCGGCGTCAGCGTCCGCCATATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTGAGACCGGGCACGGCGTCACCGTGAAGATCTCCGAGGGGTGTT
TGATCCTGATTGCAGAGACGGACGAAGTGAGGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATT
AAGGCGGGGGTAAATAATGTGGTGAACGGGGATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT205171 WP_000581937.1 NZ_CP076270:c3036571-3036323 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT205171 NZ_CP101390:c3785166-3784744 [Salmonella enterica]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCGGAGATCTTCACGGTGACGCCGTGCCCGGTCTCAAATCCCGCTT
CTTCCTGCCAGTCACCCTTAAGGTGCTGGCTGGGGATTTGTGAGTAACAGGCGGTCATGCAGGTTTCGCTGTTGATATGG
CGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTATTTATCCGCCGGATGCTGGCTGATTGTGGA
GCTCAGGGTGAGTGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCCCGGCCAGCCCCGGCCGGTGATGAGCA
GCCGGCTGCCGGGGCCGTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References