Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2750594..2751219 Replicon chromosome
Accession NZ_CP076270
Organism Escherichia coli strain Ceylon31

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag A7A14_RS13425 Protein ID WP_000911329.1
Coordinates 2750594..2750992 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag A7A14_RS13430 Protein ID WP_000450524.1
Coordinates 2750992..2751219 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A14_RS13405 (2746472) 2746472..2746672 + 201 WP_000383836.1 YpfN family protein -
A7A14_RS13410 (2746782) 2746782..2747480 - 699 WP_000679823.1 esterase -
A7A14_RS13415 (2747554) 2747554..2749569 - 2016 WP_000829293.1 tRNA cytosine(34) acetyltransferase TmcA -
A7A14_RS13420 (2749584) 2749584..2750447 - 864 WP_001267498.1 neutral zinc metallopeptidase -
A7A14_RS13425 (2750594) 2750594..2750992 - 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
A7A14_RS13430 (2750992) 2750992..2751219 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
A7A14_RS13435 (2751374) 2751374..2752087 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
A7A14_RS13440 (2752300) 2752300..2753334 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
A7A14_RS13445 (2753351) 2753351..2754229 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
A7A14_RS13450 (2754375) 2754375..2754947 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
A7A14_RS13455 (2754947) 2754947..2755417 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T205170 WP_000911329.1 NZ_CP076270:c2750992-2750594 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T205170 NZ_CP101390:3577045-3577263 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT205170 WP_000450524.1 NZ_CP076270:c2751219-2750992 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT205170 NZ_CP101390:3576643-3577017 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References