Detailed information of TA system TA205159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1589369..1590007
• Replicon: chromosome
• Accession: NZ_CP076270
• Organism: Escherichia coli strain Ceylon31

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: A7A14_RS07730
• Protein ID: WP_000813794.1
• Coordinates: 1589369..1589545 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: A7A14_RS07735
• Protein ID: WP_001270286.1
• Coordinates: 1589591..1590007 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A14_RS07710 (1584988)	1584988..1586202	-	1215	WP_001326689.1	BenE family transporter YdcO	-
A7A14_RS07715 (1586255)	1586255..1586791	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
A7A14_RS07720 (1586864)	1586864..1588825	+	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
A7A14_RS07725 (1588917)	1588917..1589147	-	231	WP_000494244.1	YncJ family protein	-
A7A14_RS07730 (1589369)	1589369..1589545	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
A7A14_RS07735 (1589591)	1589591..1590007	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
A7A14_RS07740 (1590086)	1590086..1591492	+	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
A7A14_RS07745 (1591737)	1591737..1592882	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
A7A14_RS07750 (1592900)	1592900..1593913	+	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
A7A14_RS07755 (1593914)	1593914..1594855	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1583800..1584948	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T205159 WP_000813794.1 NZ_CP076270:1589369-1589545 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T205159 NZ_CP101388:c4805891-4805580 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT205159 WP_001270286.1 NZ_CP076270:1589591-1590007 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT205159 NZ_CP101388:c4805579-4805289 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• PDB: 6HPB