Detailed information of TA system TA020515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 754753..755386
• Replicon: chromosome
• Accession: NC_002945
• Organism: Mycobacterium bovis AF2122/97

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQD6
• Locus tag: BQ2027_RS03450
• Protein ID: WP_003403365.1
• Coordinates: 754753..755136 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: BQ2027_RS03455
• Protein ID: WP_003403368.1
• Coordinates: 755231..755386 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BQ2027_RS03425 (BQ2027_MB0670)	750045..750581	+	537	WP_003403341.1	50S ribosomal protein L10	-
BQ2027_RS03430 (BQ2027_MB0671)	750618..751010	+	393	WP_003403353.1	50S ribosomal protein L7/L12	-
BQ2027_RS03435 (BQ2027_MB0672C)	751003..751698	-	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
BQ2027_RS03440 (BQ2027_MB0673)	751769..753274	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
BQ2027_RS03445 (BQ2027_MB0674)	753355..754365	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
BQ2027_RS03450 (BQ2027_MB0675C)	754753..755136	-	384	WP_003403365.1	ribonuclease VapC6	Toxin
BQ2027_RS03455 (BQ2027_MB0676C)	755231..755386	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BQ2027_RS03460 (BQ2027_MB0677C)	755462..756178	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
BQ2027_RS03465 (BQ2027_MB0678C)	756454..756762	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
BQ2027_RS03470 (BQ2027_MB0679C)	756749..756994	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
BQ2027_RS03475 (BQ2027_MB0680C)	757104..757541	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
BQ2027_RS03480 (BQ2027_MB0681C)	757538..757792	-	255	WP_003911263.1	antitoxin VapB7	-
BQ2027_RS03485 (BQ2027_MB0682)	757906..760269	+	2364	WP_003403397.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13959.73 Da
• Isoelectric Point: 6.0803

>T20515 WP_003403365.1 NC_002945:c755136-754753 [Mycobacterium tuberculosis variant bovis AF2122/97]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T20515 NC_002945:c755136-754753 [Mycobacterium tuberculosis variant bovis AF2122/97]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT20515 WP_003403368.1 NC_002945:c755386-755231 [Mycobacterium tuberculosis variant bovis AF2122/97]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT20515 NC_002945:c755386-755231 [Mycobacterium tuberculosis variant bovis AF2122/97]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Structures

Toxin

• AlphaFold DB: G0TQD6

Antitoxin

• AlphaFold DB: A0A7U4BSF8