Detailed information of TA system TA205148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 4670871..4672206
• Replicon: chromosome
• Accession: NZ_CP076268
• Organism: Escherichia coli strain D217-5

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: F4VMR9
• Locus tag: A7A16_RS22595
• Protein ID: WP_000342497.1
• Coordinates: 4671259..4672206 (+)
• Length: 316 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: E0J2C6
• Locus tag: A7A16_RS22590
• Protein ID: WP_000152741.1
• Coordinates: 4670871..4671266 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A16_RS22575 (4666835)	4666835..4668109	-	1275	WP_001293046.1	restriction endonuclease subunit S	-
A7A16_RS22580 (4668106)	4668106..4669662	-	1557	WP_000202821.1	type I restriction-modification system subunit M	-
A7A16_RS22585 (4669968)	4669968..4670801	+	834	WP_001192743.1	DUF4942 domain-containing protein	-
A7A16_RS22590 (4670871)	4670871..4671266	+	396	WP_000152741.1	DUF6088 family protein	Antitoxin
A7A16_RS22595 (4671259)	4671259..4672206	+	948	WP_000342497.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
A7A16_RS22600 (4672528)	4672528..4672671	-	144	Protein_4405	HNH endonuclease	-
A7A16_RS22605 (4672808)	4672808..4673458	+	651	WP_001037963.1	HNH endonuclease	-
A7A16_RS22610 (4673466)	4673466..4674446	-	981	WP_000991446.1	sialate O-acetylesterase	-
A7A16_RS22615 (4674511)	4674511..4675617	-	1107	WP_001298927.1	N-acetylneuraminate epimerase	-
A7A16_RS22620 (4675637)	4675637..4676353	-	717	WP_001295734.1	N-acetylneuraminic acid outer membrane channel NanC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE	4670871..4679485	8614

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-303)

Antitoxin

• Protein of unknown function DUF6088 (7-108)

Sequences

Toxin

Protein

• Length: 316 a.a.
• Molecular weight: 35588.79 Da
• Isoelectric Point: 4.9737

>T205148 WP_000342497.1 NZ_CP076268:4671259-4672206 [Escherichia coli]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYQMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA

Nucleotide

• Length: 948 bp

>T205148 NZ_CP101388:365061-365336 [Salmonella enterica]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14619.93 Da
• Isoelectric Point: 11.6915

>AT205148 WP_000152741.1 NZ_CP076268:4670871-4671266 [Escherichia coli]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRELVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG

Nucleotide

• Length: 396 bp

>AT205148 NZ_CP101388:364798-365058 [Salmonella enterica]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCCCGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Structures

Toxin

• AlphaFold DB: A0A1L5BAQ6

Antitoxin

• AlphaFold DB: A0A1L4ZXD4