205143

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3183335..3183989 Replicon chromosome
Accession NZ_CP076268
Organism Escherichia coli strain D217-5

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag A7A16_RS15470 Protein ID WP_000244772.1
Coordinates 3183335..3183742 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag A7A16_RS15475 Protein ID WP_000354046.1
Coordinates 3183723..3183989 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A16_RS15450 (3179292) 3179292..3181025 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
A7A16_RS15455 (3181031) 3181031..3181741 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
A7A16_RS15460 (3181766) 3181766..3182662 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
A7A16_RS15465 (3182774) 3182774..3183295 + 522 WP_001055874.1 flavodoxin FldB -
A7A16_RS15470 (3183335) 3183335..3183742 - 408 WP_000244772.1 protein YgfX Toxin
A7A16_RS15475 (3183723) 3183723..3183989 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
A7A16_RS15480 (3184232) 3184232..3185212 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
A7A16_RS15485 (3185465) 3185465..3186445 + 981 WP_000399648.1 IS110-like element IS621 family transposase -
A7A16_RS15490 (3186568) 3186568..3187227 - 660 WP_000250274.1 hemolysin III family protein -
A7A16_RS15495 (3187391) 3187391..3187702 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3185465..3186445 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T205143 WP_000244772.1 NZ_CP076268:c3183742-3183335 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T205143 NZ_CP101386:c4777178-4776867 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT205143 WP_000354046.1 NZ_CP076268:c3183989-3183723 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT205143 NZ_CP101386:c4776866-4776576 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QD57

References