Detailed information of TA system TA205130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1588891..1589529
• Replicon: chromosome
• Accession: NZ_CP076268
• Organism: Escherichia coli strain D217-5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: A7A16_RS07740
• Protein ID: WP_000813794.1
• Coordinates: 1588891..1589067 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: A7A16_RS07745
• Protein ID: WP_001270285.1
• Coordinates: 1589113..1589529 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A16_RS07720 (1584510)	1584510..1585724	-	1215	WP_071601296.1	BenE family transporter YdcO	-
A7A16_RS07725 (1585777)	1585777..1586313	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
A7A16_RS07730 (1586386)	1586386..1588347	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
A7A16_RS07735 (1588439)	1588439..1588669	-	231	WP_000494244.1	YncJ family protein	-
A7A16_RS07740 (1588891)	1588891..1589067	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
A7A16_RS07745 (1589113)	1589113..1589529	+	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
A7A16_RS07750 (1589608)	1589608..1591014	+	1407	WP_000760594.1	PLP-dependent aminotransferase family protein	-
A7A16_RS07755 (1591259)	1591259..1592404	+	1146	WP_039022646.1	ABC transporter substrate-binding protein	-
A7A16_RS07760 (1592422)	1592422..1593435	+	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
A7A16_RS07765 (1593436)	1593436..1594377	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1583322..1584470	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T205130 WP_000813794.1 NZ_CP076268:1588891-1589067 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T205130 NZ_CP101386:206805-207290 [Salmonella enterica]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT205130 WP_001270285.1 NZ_CP076268:1589113-1589529 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT205130 NZ_CP101386:206530-206817 [Salmonella enterica]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• PDB: 6HPB