Detailed information of TA system TA020513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 717656..718305
• Replicon: chromosome
• Accession: NC_002945
• Organism: Mycobacterium bovis AF2122/97

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67241
• Locus tag: BQ2027_RS03265
• Protein ID: WP_003403236.1
• Coordinates: 717910..718305 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ34
• Locus tag: BQ2027_RS03260
• Protein ID: WP_003403235.1
• Coordinates: 717656..717910 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BQ2027_RS03235	712781..712864	+	84	Protein_648	galactose-1-phosphate uridylyltransferase	-
BQ2027_RS03240 (BQ2027_MB0635)	712883..713965	+	1083	WP_031702118.1	galactose-1-phosphate uridylyltransferase	-
BQ2027_RS03245 (BQ2027_MB0636)	713962..715053	+	1092	WP_003403225.1	galactokinase	-
BQ2027_RS03250 (BQ2027_MB0637)	715448..716605	+	1158	WP_003903091.1	hypothetical protein	-
BQ2027_RS03255 (BQ2027_MB0638)	716616..717563	+	948	WP_003403232.1	DUF732 domain-containing protein	-
BQ2027_RS03260 (BQ2027_MB0639)	717656..717910	+	255	WP_003403235.1	type II toxin-antitoxin system antitoxin VapB30	Antitoxin
BQ2027_RS03265 (BQ2027_MB0640)	717910..718305	+	396	WP_003403236.1	type II toxin-antitoxin system toxin ribonuclease C30	Toxin
BQ2027_RS03270 (BQ2027_MB0641C)	718399..719139	-	741	WP_003403239.1	TVP38/TMEM64 family protein	-
BQ2027_RS03275 (BQ2027_MB0642)	719271..719531	+	261	WP_003403244.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BQ2027_RS03280 (BQ2027_MB0643)	719528..719935	+	408	WP_003403246.1	type II toxin-antitoxin system VapC family toxin	-
BQ2027_RS03285 (BQ2027_MB0644C)	720007..721158	-	1152	WP_003403248.1	FIST N-terminal domain-containing protein	-
BQ2027_RS03290 (BQ2027_MB0645C)	721251..722978	-	1728	WP_010950423.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14250.04 Da
• Isoelectric Point: 4.7475

>T20513 WP_003403236.1 NC_002945:717910-718305 [Mycobacterium tuberculosis variant bovis AF2122/97]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Nucleotide

• Length: 396 bp

>T20513 NC_002945:717910-718305 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9139.52 Da
• Isoelectric Point: 9.2702

>AT20513 WP_003403235.1 NC_002945:717656-717910 [Mycobacterium tuberculosis variant bovis AF2122/97]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Nucleotide

• Length: 255 bp

>AT20513 NC_002945:717656-717910 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Structures

Toxin

• PDB: 4XGR
• PDB: 4XGQ

Antitoxin

• AlphaFold DB: A0A7U4BSC2