Detailed information of TA system TA205124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 496341..497178
• Replicon: chromosome
• Accession: NZ_CP076268
• Organism: Escherichia coli strain D217-5

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: A7A16_RS02350
• Protein ID: WP_000227784.1
• Coordinates: 496341..496883 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: A7A16_RS02355
• Protein ID: WP_001297137.1
• Coordinates: 496867..497178 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A16_RS02330 (491880)	491880..492791	-	912	WP_000705849.1	2-dehydropantoate 2-reductase	-
A7A16_RS02335 (492959)	492959..493450	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
A7A16_RS02340 (493578)	493578..494942	-	1365	WP_001000978.1	MFS transporter	-
A7A16_RS02345 (495350)	495350..496285	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
A7A16_RS02350 (496341)	496341..496883	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
A7A16_RS02355 (496867)	496867..497178	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
A7A16_RS02360 (497363)	497363..498253	-	891	WP_000971336.1	heme o synthase	-
A7A16_RS02365 (498265)	498265..498594	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
A7A16_RS02370 (498594)	498594..499208	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
A7A16_RS02375 (499198)	499198..501189	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
A7A16_RS02380 (501211)	501211..502158	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T205124 WP_000227784.1 NZ_CP076268:c496883-496341 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T205124 NZ_CP101382:c4176999-4176712 [Salmonella enterica]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT205124 WP_001297137.1 NZ_CP076268:c497178-496867 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT205124 NZ_CP101382:c4176725-4176423 [Salmonella enterica]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3