Detailed information of TA system TA020512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 712027..712652
• Replicon: chromosome
• Accession: NC_002945
• Organism: Mycobacterium bovis AF2122/97

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQA0
• Locus tag: BQ2027_RS03230
• Protein ID: WP_003403218.1
• Coordinates: 712251..712652 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ36
• Locus tag: BQ2027_RS03225
• Protein ID: WP_003403213.1
• Coordinates: 712027..712254 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BQ2027_RS03200	707206..707427	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
BQ2027_RS03205 (BQ2027_MB0629)	707569..708174	+	606	WP_003898526.1	hypothetical protein	-
BQ2027_RS03210 (BQ2027_MB0630C)	708193..710760	-	2568	WP_003900188.1	SEC-C metal-binding domain-containing protein	-
BQ2027_RS03215 (BQ2027_MB0631)	710844..711593	+	750	WP_003898528.1	hypothetical protein	-
BQ2027_RS03220 (BQ2027_MB0632)	711590..711832	+	243	WP_003403210.1	hypothetical protein	-
BQ2027_RS03225 (BQ2027_MB0633A)	712027..712254	+	228	WP_003403213.1	antitoxin VapB29	Antitoxin
BQ2027_RS03230 (BQ2027_MB0634)	712251..712652	+	402	WP_003403218.1	type II toxin-antitoxin system ribonuclease VapC29	Toxin
BQ2027_RS03235	712781..712864	+	84	Protein_648	galactose-1-phosphate uridylyltransferase	-
BQ2027_RS03240 (BQ2027_MB0635)	712883..713965	+	1083	WP_031702118.1	galactose-1-phosphate uridylyltransferase	-
BQ2027_RS03245 (BQ2027_MB0636)	713962..715053	+	1092	WP_003403225.1	galactokinase	-
BQ2027_RS03250 (BQ2027_MB0637)	715448..716605	+	1158	WP_003903091.1	hypothetical protein	-
BQ2027_RS03255 (BQ2027_MB0638)	716616..717563	+	948	WP_003403232.1	DUF732 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-120)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 13937.76 Da
• Isoelectric Point: 5.7441

>T20512 WP_003403218.1 NC_002945:712251-712652 [Mycobacterium tuberculosis variant bovis AF2122/97]
VTVLLDANVLIALVVAEHVHHDAAADWLMASDTGFATCPMTQGSLVRFLVRSGQSAAAARDVVSAVQCTSRHEFWPDALS
FAGVEVAGVVGHRQVTDAYLAQLARSHDGQLATLDSGLAHLHGDVAVLIPTTT

Nucleotide

• Length: 402 bp

>T20512 NC_002945:712251-712652 [Mycobacterium tuberculosis variant bovis AF2122/97]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGAGCATGTGCATCATGATGCTGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCCAACGACCACCT
GA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8163.17 Da
• Isoelectric Point: 5.6307

>AT20512 WP_003403213.1 NC_002945:712027-712254 [Mycobacterium tuberculosis variant bovis AF2122/97]
VRTTIDLPQDLHKQALAIARDTHRTLSETVADLMRRGLAANRPTALSSDPRTGLPLVSVGTVVTSEDVRSLEDEQ

Nucleotide

• Length: 228 bp

>AT20512 NC_002945:712027-712254 [Mycobacterium tuberculosis variant bovis AF2122/97]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGCCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA

Structures

Toxin

• AlphaFold DB: G0TQA0

Antitoxin

• AlphaFold DB: A0A7U4BSC9