Detailed information of TA system TA205118

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 62579..63204
• Replicon: plasmid pNADC1036IncF
• Accession: NZ_CP076265
• Organism: Escherichia coli strain NADC1036

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A7A17_RS24200
• Protein ID: WP_000911317.1
• Coordinates: 62806..63204 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: A7A17_RS24195
• Protein ID: WP_000450532.1
• Coordinates: 62579..62806 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A17_RS24195 (62579)	62579..62806	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
A7A17_RS24200 (62806)	62806..63204	+	399	WP_000911317.1	type II toxin-antitoxin system VapC family toxin	Toxin
A7A17_RS24205 (63213)	63213..65366	-	2154	WP_001603683.1	type IV conjugative transfer system coupling protein TraD	-
A7A17_RS24210 (65618)	65618..66328	-	711	WP_230016160.1	conjugal transfer complement resistance protein TraT	-
A7A17_RS24215 (66363)	66363..66872	-	510	WP_000628105.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cdtC / cdtB / cdtA / cnf1 / hlyC / iutA / iucD / iucC / iucB / iucA / f17d-G / f17d-C / f17d-D / f17d-A	1..145019	145019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14857.14 Da
• Isoelectric Point: 8.5264

>T205118 WP_000911317.1 NZ_CP076265:62806-63204 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T205118 NZ_CP101382:930182-930580 [Salmonella enterica]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT205118 WP_000450532.1 NZ_CP076265:62579-62806 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT205118 NZ_CP101382:929955-930182 [Salmonella enterica]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5