Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3849612..3850412 Replicon chromosome
Accession NZ_CP076264
Organism Escherichia coli strain NADC1036

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag A7A17_RS19035 Protein ID WP_000342449.1
Coordinates 3849612..3850139 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag A7A17_RS19040 Protein ID WP_001277108.1
Coordinates 3850146..3850412 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A17_RS19010 (3844687) 3844687..3845454 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
A7A17_RS19015 (3845451) 3845451..3846728 - 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
A7A17_RS19020 (3846725) 3846725..3847651 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
A7A17_RS19025 (3847699) 3847699..3848808 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
A7A17_RS19030 (3849232) 3849232..3849615 + 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
A7A17_RS19035 (3849612) 3849612..3850139 - 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
A7A17_RS19040 (3850146) 3850146..3850412 - 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
A7A17_RS19045 (3850562) 3850562..3851665 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
A7A17_RS19050 (3851936) 3851936..3852790 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
A7A17_RS19055 (3853035) 3853035..3854093 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
A7A17_RS19060 (3854086) 3854086..3854754 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T205109 WP_000342449.1 NZ_CP076264:c3850139-3849612 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T205109 NZ_CP101379:c4229941-4229657 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT205109 WP_001277108.1 NZ_CP076264:c3850412-3850146 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT205109 NZ_CP101379:c4230173-4229931 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References