Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3275556..3276210 Replicon chromosome
Accession NZ_CP076264
Organism Escherichia coli strain NADC1036

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag A7A17_RS16150 Protein ID WP_000244772.1
Coordinates 3275556..3275963 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag A7A17_RS16155 Protein ID WP_000354046.1
Coordinates 3275944..3276210 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A17_RS16130 (3271513) 3271513..3273246 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
A7A17_RS16135 (3273252) 3273252..3273962 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
A7A17_RS16140 (3273987) 3273987..3274883 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
A7A17_RS16145 (3274995) 3274995..3275516 + 522 WP_001055874.1 flavodoxin FldB -
A7A17_RS16150 (3275556) 3275556..3275963 - 408 WP_000244772.1 protein YgfX Toxin
A7A17_RS16155 (3275944) 3275944..3276210 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
A7A17_RS16160 (3276453) 3276453..3277433 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
A7A17_RS16165 (3277510) 3277510..3278169 - 660 WP_000250274.1 hemolysin III family protein -
A7A17_RS16170 (3278333) 3278333..3278644 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
A7A17_RS16175 (3278689) 3278689..3280122 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
A7A17_RS16180 (3280179) 3280179..3280922 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T205107 WP_000244772.1 NZ_CP076264:c3275963-3275556 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T205107 NZ_CP101379:3724742-3725017 [Salmonella enterica]
ATGAATGCCTCCGGCGTCAGCGTCCGCCATATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTGAGACCGGGCACGGCGTCACCGTGAAGATCTCCGAGGGGTGTT
TGATCCTGATTGCAGAGACGGACGAAGTGAGGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATT
AAGGCGGGGGTAAATAATGTGGTGAACGGGGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT205107 WP_000354046.1 NZ_CP076264:c3276210-3275944 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT205107 NZ_CP101379:c3725007-3724585 [Salmonella enterica]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCGGAGATCTTCACGGTGACGCCGTGCCCGGTCTCAAATCCCGCTT
CTTCCTGCCAGTCACCCTTAAGGTGCTGGCTGGGGATTTGTGAGTAACAGGCGGTCATGCAGGTTTCGCTGTTGATATGG
CGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTATTTATCCGCCGGATGCTGGCTGATTGTGGA
GCTCAGGGTGAGTGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCCCGGCCAGCCCCGGCCGGTGATGAGCA
GCCGGCTGCCGGGGCCGTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References