Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 516869..517706 Replicon chromosome
Accession NZ_CP076264
Organism Escherichia coli strain NADC1036

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag A7A17_RS02520 Protein ID WP_000227784.1
Coordinates 516869..517411 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag A7A17_RS02525 Protein ID WP_001297137.1
Coordinates 517395..517706 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A17_RS02500 (512408) 512408..513319 - 912 WP_000705853.1 2-dehydropantoate 2-reductase -
A7A17_RS02505 (513487) 513487..513978 + 492 WP_001138904.1 nucleotide binding protein YajQ -
A7A17_RS02510 (514106) 514106..515470 - 1365 WP_001000978.1 MFS transporter -
A7A17_RS02515 (515878) 515878..516813 + 936 WP_001297127.1 tetratricopeptide repeat protein -
A7A17_RS02520 (516869) 516869..517411 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
A7A17_RS02525 (517395) 517395..517706 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
A7A17_RS02530 (517891) 517891..518781 - 891 WP_000971336.1 heme o synthase -
A7A17_RS02535 (518793) 518793..519122 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
A7A17_RS02540 (519122) 519122..519736 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
A7A17_RS02545 (519726) 519726..521717 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
A7A17_RS02550 (521739) 521739..522686 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T205088 WP_000227784.1 NZ_CP076264:c517411-516869 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T205088 NZ_CP101376:2128397-2128500 [Salmonella enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT205088 WP_001297137.1 NZ_CP076264:c517706-517395 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT205088 NZ_CP101376:c2128502-2128357 [Salmonella enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References