Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 66008..66811 Replicon plasmid p04IncF
Accession NZ_CP076263
Organism Escherichia coli strain 0-4

Toxin (Protein)


Gene name KacT Uniprot ID A0A0K3X5E3
Locus tag A7A18_RS24795 Protein ID WP_000348884.1
Coordinates 66008..66538 (-) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID G3CAI7
Locus tag A7A18_RS24800 Protein ID WP_001275013.1
Coordinates 66542..66811 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A18_RS24765 (61261) 61261..62214 + 954 WP_001068902.1 ferric citrate uptake sigma factor regulator FecR -
A7A18_RS24770 (62301) 62301..64625 + 2325 WP_021534628.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
A7A18_RS24775 (64670) 64670..64757 + 88 Protein_74 hypothetical protein -
A7A18_RS24780 (64828) 64828..65100 + 273 WP_000019163.1 hypothetical protein -
A7A18_RS24785 (65081) 65081..65731 + 651 Protein_76 transposase zinc-binding domain-containing protein -
A7A18_RS24790 (65790) 65790..66007 - 218 Protein_77 transposase -
A7A18_RS24795 (66008) 66008..66538 - 531 WP_000348884.1 GNAT family N-acetyltransferase Toxin
A7A18_RS24800 (66542) 66542..66811 - 270 WP_001275013.1 DUF1778 domain-containing protein Antitoxin
A7A18_RS24805 (67699) 67699..68688 + 990 WP_024201093.1 RepB family plasmid replication initiator protein -
A7A18_RS24810 (68971) 68971..69711 - 741 WP_021534629.1 tyrosine-type recombinase/integrase -
A7A18_RS24815 (69832) 69832..69966 - 135 Protein_82 serine/threonine protein kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..77652 77652
- flank IS/Tn - - 65081..65446 365


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20320.22 Da        Isoelectric Point: 6.7486

>T205086 WP_000348884.1 NZ_CP076263:c66538-66008 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEVLFEVNDE

Download         Length: 531 bp

>T205086 NZ_CP101376:881417-881815 [Salmonella enterica]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10229.63 Da        Isoelectric Point: 6.4702

>AT205086 WP_001275013.1 NZ_CP076263:c66811-66542 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Download         Length: 270 bp

>AT205086 NZ_CP101376:881190-881417 [Salmonella enterica]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K3X5E3

Antitoxin

Source ID Structure
AlphaFold DB G3CAI7

References