Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3880014..3880645 Replicon chromosome
Accession NZ_CP076262
Organism Escherichia coli strain 0-4

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag A7A18_RS19115 Protein ID WP_001259388.1
Coordinates 3880370..3880645 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag A7A18_RS19110 Protein ID WP_000593555.1
Coordinates 3880014..3880373 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A18_RS19085 (3876146) 3876146..3877090 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
A7A18_RS19090 (3877087) 3877087..3877920 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
A7A18_RS19095 (3877920) 3877920..3878684 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
A7A18_RS19100 (3878681) 3878681..3879487 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
A7A18_RS19105 (3879493) 3879493..3879894 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
A7A18_RS19110 (3880014) 3880014..3880373 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
A7A18_RS19115 (3880370) 3880370..3880645 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
A7A18_RS19120 (3880718) 3880718..3881842 - 1125 WP_001216257.1 ABC transporter permease -
A7A18_RS19125 (3881842) 3881842..3884577 - 2736 WP_000149124.1 ribosome-associated ATPase/putative transporter RbbA -
A7A18_RS19130 (3884574) 3884574..3885641 - 1068 WP_096974684.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T205077 WP_001259388.1 NZ_CP076262:c3880645-3880370 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T205077 NZ_CP101375:4155205-4155423 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT205077 WP_000593555.1 NZ_CP076262:c3880373-3880014 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT205077 NZ_CP101375:4154803-4155177 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References