Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3276701..3277355 Replicon chromosome
Accession NZ_CP076262
Organism Escherichia coli strain 0-4

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag A7A18_RS16060 Protein ID WP_000244772.1
Coordinates 3276701..3277108 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag A7A18_RS16065 Protein ID WP_000354046.1
Coordinates 3277089..3277355 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A18_RS16040 (3272658) 3272658..3274391 - 1734 WP_096974661.1 single-stranded-DNA-specific exonuclease RecJ -
A7A18_RS16045 (3274397) 3274397..3275107 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
A7A18_RS16050 (3275132) 3275132..3276028 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
A7A18_RS16055 (3276140) 3276140..3276661 + 522 WP_001055874.1 flavodoxin FldB -
A7A18_RS16060 (3276701) 3276701..3277108 - 408 WP_000244772.1 protein YgfX Toxin
A7A18_RS16065 (3277089) 3277089..3277355 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
A7A18_RS16070 (3277598) 3277598..3278578 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
A7A18_RS16075 (3278655) 3278655..3279314 - 660 WP_000250274.1 hemolysin III family protein -
A7A18_RS16080 (3279478) 3279478..3279789 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
A7A18_RS16085 (3279834) 3279834..3281267 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
A7A18_RS16090 (3281324) 3281324..3282067 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T205075 WP_000244772.1 NZ_CP076262:c3277108-3276701 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T205075 NZ_CP101375:2764559-2764662 [Salmonella enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT205075 WP_000354046.1 NZ_CP076262:c3277355-3277089 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT205075 NZ_CP101375:c2764664-2764519 [Salmonella enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References