Detailed information of TA system TA205062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1620752..1621390
• Replicon: chromosome
• Accession: NZ_CP076262
• Organism: Escherichia coli strain 0-4

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: A7A18_RS07910
• Protein ID: WP_000813794.1
• Coordinates: 1620752..1620928 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: A7A18_RS07915
• Protein ID: WP_001270286.1
• Coordinates: 1620974..1621390 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A18_RS07890 (1616371)	1616371..1617585	-	1215	WP_001326689.1	BenE family transporter YdcO	-
A7A18_RS07895 (1617638)	1617638..1618174	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
A7A18_RS07900 (1618247)	1618247..1620208	+	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
A7A18_RS07905 (1620300)	1620300..1620530	-	231	WP_000494244.1	YncJ family protein	-
A7A18_RS07910 (1620752)	1620752..1620928	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
A7A18_RS07915 (1620974)	1620974..1621390	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
A7A18_RS07920 (1621469)	1621469..1622875	+	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
A7A18_RS07925 (1623120)	1623120..1624265	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
A7A18_RS07930 (1624283)	1624283..1625296	+	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
A7A18_RS07935 (1625297)	1625297..1626238	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1615183..1616331	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T205062 WP_000813794.1 NZ_CP076262:1620752-1620928 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T205062 NZ_CP101373:c4754535-4754224 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT205062 WP_001270286.1 NZ_CP076262:1620974-1621390 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT205062 NZ_CP101373:c4754223-4753933 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• PDB: 6HPB