Detailed information of TA system TA020506

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 364873..365516
• Replicon: chromosome
• Accession: NC_002945
• Organism: Mycobacterium bovis AF2122/97

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB8
• Locus tag: BQ2027_RS01605
• Protein ID: WP_003401566.1
• Coordinates: 365091..365516 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: BQ2027_RS01600
• Protein ID: WP_003401563.1
• Coordinates: 364873..365094 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BQ2027_RS01575 (BQ2027_MB0303C)	359947..360750	-	804	WP_003401540.1	Stf0 family sulphotransferase	-
BQ2027_RS01580 (BQ2027_MB0304C)	360760..362157	-	1398	WP_003401544.1	sulfatase	-
BQ2027_RS01585 (BQ2027_MB0305)	362336..364156	+	1821	WP_003401546.1	PE family protein	-
BQ2027_RS01590 (BQ2027_MB0306)	364299..364526	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
BQ2027_RS01595 (BQ2027_MB0307)	364523..364825	+	303	WP_010950376.1	hypothetical protein	-
BQ2027_RS01600 (BQ2027_MB0308)	364873..365094	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
BQ2027_RS01605 (BQ2027_MB0309)	365091..365516	+	426	WP_003401566.1	PIN domain nuclease	Toxin
BQ2027_RS01610 (BQ2027_MB0310)	365652..366284	+	633	WP_010950377.1	TetR/AcrR family transcriptional regulator	-
BQ2027_RS01615 (BQ2027_MB0311)	366281..367189	+	909	WP_003900117.1	protochlorophyllide reductase	-
BQ2027_RS20165	367197..367787	-	591	WP_234712434.1	hypothetical protein	-
BQ2027_RS20170	367780..367830	-	51	Protein_321	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-125)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15713.90 Da
• Isoelectric Point: 5.6002

>T20506 WP_003401566.1 NC_002945:365091-365516 [Mycobacterium tuberculosis variant bovis AF2122/97]
VTDQRWLIDKSALVRLTDSPDMEIWSNRIERGLVHITGVTRLEVGFSAECGEIARREFREPPLSAMPVEYLTPRIEDRAL
EVQTLLADRGHHRGPSIPDLLIAATAELSGLTVLHVDKDFDAIAALTGQKTERLTHRPPSA

Nucleotide

• Length: 426 bp

>T20506 NC_002945:365091-365516 [Mycobacterium tuberculosis variant bovis AF2122/97]
GTGACTGACCAGCGCTGGCTGATCGACAAGTCGGCGCTGGTGCGGCTCACGGACAGCCCTGACATGGAAATCTGGTCGAA
CCGGATCGAACGCGGCCTGGTACACATCACGGGCGTGACACGCTTGGAAGTAGGGTTCTCGGCCGAATGCGGGGAGATAG
CGCGACGGGAGTTTCGTGAACCGCCGCTGTCTGCGATGCCCGTGGAATACCTAACCCCGAGAATTGAAGACCGTGCGCTC
GAGGTGCAGACCTTGCTTGCCGACCGCGGACACCACCGTGGCCCGTCGATCCCGGATCTGCTCATCGCCGCGACAGCCGA
ACTGTCGGGCTTGACGGTACTGCACGTCGACAAGGACTTTGACGCCATCGCCGCGCTTACCGGTCAGAAAACAGAACGGC
TCACGCATCGCCCGCCTTCCGCTTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT20506 WP_003401563.1 NC_002945:364873-365094 [Mycobacterium tuberculosis variant bovis AF2122/97]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT20506 NC_002945:364873-365094 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Toxin

• PDB: 3H87

Antitoxin

• AlphaFold DB: A0A829CG48