Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 84158..84801 Replicon plasmid pSA31IncI2
Accession NZ_CP076261
Organism Escherichia coli strain WY517-2

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag A7A20_RS24175 Protein ID WP_257115067.1
Coordinates 84385..84801 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A627FA37
Locus tag A7A20_RS24170 Protein ID WP_001604445.1
Coordinates 84158..84388 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A20_RS24140 (79377) 79377..79793 - 417 WP_001278818.1 plasmid partitioning/stability family protein -
A7A20_RS24145 (79786) 79786..80766 - 981 WP_000688514.1 plasmid segregation protein ParM -
A7A20_RS24150 (81180) 81180..81488 - 309 WP_000030199.1 hypothetical protein -
A7A20_RS24155 (81575) 81575..82219 - 645 WP_001144036.1 ParA family protein -
A7A20_RS24160 (82399) 82399..83178 - 780 WP_001164201.1 site-specific integrase -
A7A20_RS24165 (83180) 83180..83593 - 414 WP_000465043.1 hypothetical protein -
A7A20_RS24170 (84158) 84158..84388 + 231 WP_001604445.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
A7A20_RS24175 (84385) 84385..84801 + 417 WP_257115067.1 type II toxin-antitoxin system VapC family toxin Toxin
A7A20_RS24180 (84971) 84971..86371 - 1401 Protein_96 AAA family ATPase -
A7A20_RS24185 (86403) 86403..87995 - 1593 WP_257115068.1 IS66 family transposase -
A7A20_RS24190 (88026) 88026..88376 - 351 WP_257115069.1 IS66 family insertion sequence element accessory protein TnpB -
A7A20_RS24195 (88373) 88373..88807 - 435 WP_188926466.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - basG 1..115220 115220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15179.58 Da        Isoelectric Point: 6.7132

>T205055 WP_257115067.1 NZ_CP076261:84385-84801 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTKEFERVPDLVLEDWVK

Download         Length: 417 bp

>T205055 NZ_CP101373:2161288-2161391 [Salmonella enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8736.84 Da        Isoelectric Point: 4.4692

>AT205055 WP_001604445.1 NZ_CP076261:84158-84388 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVISDEGRFNL

Download         Length: 231 bp

>AT205055 NZ_CP101373:c2161393-2161248 [Salmonella enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A627FA37

References