Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 51531..52056 Replicon plasmid pWY517IncF
Accession NZ_CP076260
Organism Escherichia coli strain WY517-2

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag A7A20_RS23545 Protein ID WP_001159871.1
Coordinates 51531..51836 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag A7A20_RS23550 Protein ID WP_000813634.1
Coordinates 51838..52056 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A20_RS23515 (46798) 46798..48068 + 1271 Protein_59 translesion error-prone DNA polymerase V subunit UmuC -
A7A20_RS23520 (48073) 48073..48465 - 393 WP_000340833.1 plasmid partitioning/stability family protein -
A7A20_RS23525 (48470) 48470..49441 - 972 WP_001103694.1 plasmid segregation protein ParM -
A7A20_RS23530 (49670) 49670..50314 + 645 WP_000633911.1 AAA family ATPase -
A7A20_RS23535 (50308) 50308..50583 + 276 WP_000239529.1 hypothetical protein -
A7A20_RS23540 (50721) 50721..51530 - 810 WP_032219120.1 site-specific integrase -
A7A20_RS23545 (51531) 51531..51836 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
A7A20_RS23550 (51838) 51838..52056 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
A7A20_RS23555 (52610) 52610..55216 - 2607 WP_257120090.1 hypothetical protein -
A7A20_RS23560 (55335) 55335..56563 + 1229 WP_102384962.1 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..89049 89049


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T205050 WP_001159871.1 NZ_CP076260:c51836-51531 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T205050 NZ_CP101373:316725-317093 [Salmonella enterica]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT205050 WP_000813634.1 NZ_CP076260:c52056-51838 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT205050 NZ_CP101373:316507-316728 [Salmonella enterica]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGGCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References