205050

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 51531..52056 Replicon plasmid pWY517IncF
Accession NZ_CP076260
Organism Escherichia coli strain WY517-2

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag A7A20_RS23545 Protein ID WP_001159871.1
Coordinates 51531..51836 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag A7A20_RS23550 Protein ID WP_000813634.1
Coordinates 51838..52056 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A20_RS23515 (46798) 46798..48068 + 1271 Protein_59 translesion error-prone DNA polymerase V subunit UmuC -
A7A20_RS23520 (48073) 48073..48465 - 393 WP_000340833.1 plasmid partitioning/stability family protein -
A7A20_RS23525 (48470) 48470..49441 - 972 WP_001103694.1 plasmid segregation protein ParM -
A7A20_RS23530 (49670) 49670..50314 + 645 WP_000633911.1 AAA family ATPase -
A7A20_RS23535 (50308) 50308..50583 + 276 WP_000239529.1 hypothetical protein -
A7A20_RS23540 (50721) 50721..51530 - 810 WP_032219120.1 site-specific integrase -
A7A20_RS23545 (51531) 51531..51836 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
A7A20_RS23550 (51838) 51838..52056 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
A7A20_RS23555 (52610) 52610..55216 - 2607 WP_257120090.1 hypothetical protein -
A7A20_RS23560 (55335) 55335..56563 + 1229 WP_102384962.1 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..89049 89049


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T205050 WP_001159871.1 NZ_CP076260:c51836-51531 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T205050 NZ_CP101373:316725-317093 [Salmonella enterica]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT205050 WP_000813634.1 NZ_CP076260:c52056-51838 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT205050 NZ_CP101373:316507-316728 [Salmonella enterica]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGGCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References