Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4653313..4653908 Replicon chromosome
Accession NZ_CP076259
Organism Escherichia coli strain WY517-2

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag A7A20_RS22460 Protein ID WP_000239579.1
Coordinates 4653558..4653908 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag A7A20_RS22455 Protein ID WP_001223208.1
Coordinates 4653313..4653564 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A20_RS22445 (4648977) 4648977..4652756 + 3780 WP_000060901.1 autotransporter assembly complex protein TamB -
A7A20_RS22450 (4652759) 4652759..4653100 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
A7A20_RS22455 (4653313) 4653313..4653564 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
A7A20_RS22460 (4653558) 4653558..4653908 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
A7A20_RS22465 (4653988) 4653988..4654518 - 531 WP_000055072.1 inorganic diphosphatase -
A7A20_RS22470 (4654828) 4654828..4655784 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
A7A20_RS22475 (4656094) 4656094..4657596 + 1503 WP_000205793.1 sugar ABC transporter ATP-binding protein -
A7A20_RS22480 (4657610) 4657610..4658632 + 1023 WP_001317446.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T205047 WP_000239579.1 NZ_CP076259:4653558-4653908 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T205047 NZ_CP101372:c5048669-5048358 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT205047 WP_001223208.1 NZ_CP076259:4653313-4653564 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT205047 NZ_CP101372:c5048357-5048067 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References