Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3786290..3786876 Replicon chromosome
Accession NZ_CP076259
Organism Escherichia coli strain WY517-2

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag A7A20_RS18465 Protein ID WP_042014162.1
Coordinates 3786646..3786876 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag A7A20_RS18460 Protein ID WP_000593555.1
Coordinates 3786290..3786649 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A20_RS18435 (3782422) 3782422..3783366 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
A7A20_RS18440 (3783363) 3783363..3784196 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
A7A20_RS18445 (3784196) 3784196..3784960 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
A7A20_RS18450 (3784957) 3784957..3785763 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
A7A20_RS18455 (3785769) 3785769..3786170 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
A7A20_RS18460 (3786290) 3786290..3786649 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
A7A20_RS18465 (3786646) 3786646..3786876 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
A7A20_RS18470 (3786980) 3786980..3788104 - 1125 WP_001314210.1 ABC transporter permease -
A7A20_RS18475 (3788104) 3788104..3790839 - 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T205044 WP_042014162.1 NZ_CP076259:c3786876-3786646 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T205044 NZ_CP101372:c4464114-4463827 [Salmonella enterica]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT205044 WP_000593555.1 NZ_CP076259:c3786649-3786290 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT205044 NZ_CP101372:c4463840-4463538 [Salmonella enterica]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References