Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3764783..3765583 Replicon chromosome
Accession NZ_CP076259
Organism Escherichia coli strain WY517-2

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag A7A20_RS18340 Protein ID WP_000342449.1
Coordinates 3764783..3765310 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag A7A20_RS18345 Protein ID WP_001277108.1
Coordinates 3765317..3765583 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A20_RS18315 (3759858) 3759858..3760625 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
A7A20_RS18320 (3760622) 3760622..3761899 - 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
A7A20_RS18325 (3761896) 3761896..3762822 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
A7A20_RS18330 (3762870) 3762870..3763979 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
A7A20_RS18335 (3764403) 3764403..3764786 + 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
A7A20_RS18340 (3764783) 3764783..3765310 - 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
A7A20_RS18345 (3765317) 3765317..3765583 - 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
A7A20_RS18350 (3765733) 3765733..3766836 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
A7A20_RS18355 (3767107) 3767107..3767961 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
A7A20_RS18360 (3768206) 3768206..3769264 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
A7A20_RS18365 (3769257) 3769257..3769925 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T205043 WP_000342449.1 NZ_CP076259:c3765310-3764783 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T205043 NZ_CP101372:4052876-4053151 [Salmonella enterica]
ATGAATGCCTCCGGCGTCAGCGTCCGCCATATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTGAGACCGGGCACGGCGTCACCGTGAAGATCTCCGAGGGGTGTT
TGATCCTGATTGCAGAGACGGACGAAGTGAGGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATT
AAGGCGGGGGTAAATAATGTGGTGAACGGGGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT205043 WP_001277108.1 NZ_CP076259:c3765583-3765317 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT205043 NZ_CP101372:c4053141-4052719 [Salmonella enterica]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCGGAGATCTTCACGGTGACGCCGTGCCCGGTCTCAAATCCCGCTT
CTTCCTGCCAGTCACCCTTAAGGTGCTGGCTGGGGATTTGTGAGTAACAGGCGGTCATGCAGGTTTCGCTGTTGATATGG
CGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTATTTATCCGCCGGATGCTGGCTGATTGTGGA
GCTCAGGGTGAGTGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCCCGGCCAGCCCCGGCCGGTGATGAGCA
GCCGGCTGCCGGGGCCGTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References